本?文之研究，旨在探討台灣本土菊花，杭菊 ( Chrysanthemum morifolium Ramet ) 及野菊（Chrysanthemum indicum )之抗癌能力。以乙酸乙酯、甲醇及水三種溶劑之菊花萃取物分析對於人類肝癌Huh 7細胞及肺腺癌A549細胞之毒殺作用。結果得知乙酸乙酯萃取物效果最佳；細胞毒性試驗顯示，野菊萃取物優於杭菊萃取物，而且抑制癌細胞增生的效力是有選擇性的；癌細胞經野菊萃取物處理後，其caspase-3被活化，再透過流式細胞儀分析及DAPI染色顯影，證實?野菊萃取物會誘發癌細胞細胞凋亡。菊花萃取物可選擇性的抑制 Huh 7細胞和A549細胞之生長，或促使此兩株細胞死亡，並探討細胞的死亡是否循細胞凋亡的路徑。由實驗結果中也證實了野菊（Chrysanthemum indicum ) 萃取物抗癌能力優於杭菊 ( Chrysanthemum morifolium Ramet ) 萃取物。而萃取溶劑方面，乙酸乙酯萃取有效物質之效力最佳，甲醇次之，水為最差。實驗中我們也利用正常的人類臍靜脈內皮細胞同樣進行毒性測試，發現菊花乙酸乙酯萃取物選擇性地對於癌化細胞具毒殺效果，對於正常細胞毒性甚低。另外，也發現經野菊乙酸乙酯萃取物處理的癌細胞，其caspase-3被活化，再透過流式細胞儀分析及DAPI染色顯影，證實?其死亡現象為細胞凋亡。由本?文研究，讓我們?進一步的瞭解菊花之抗癌活性。由實驗結果得知，菊花具潛?開發為抗肺腺癌及肝癌之藥物，未?應?深入的研究，提供菊花應用在抗癌活性上之?考。 The aim of this study is to explore the anticancer activity of ingredients isolated from local Harng Jyur (Chrysanthemum morifolium Ramet ) and Yeh Jyur ( Chrysanthemum indicum) flowers. Flower extracts were prepared by using ethyl acetate, methanol, and water. Among these three various extracts, the ethyl acetate extracts displayed the best potency to kill human hepatoma Huh 7 cells and human adenocarcinoma A549 cells. The cytotoxicity of Yeh Jyur extracts is superior to that of Harng Jyur, and exhibited anti-proliferation against cancer cells with selectivity. Treatment f cancer cells with flower extracts resulted in apoptotic cell death as evaluated by the morphological change, nuclear condensation and subG1 population increasing. Moreover, the tested extracts induced caspase-3 activation, indicating that extracts induced partly a caspase-dependent apoptotic cell death. Our observations suggest that the compounds of chrysanthemum flowers with anticancer efficacy are worthwhile to identify for the purpose of developing prodrugs of chemotherapy.
