固態發酵培養基組成對於樟芝菌絲體活性成份生成之影響

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Title:	固態發酵培養基組成對於樟芝菌絲體活性成份生成之影響
Other Titles:	Effect of medium compositionon on the fermentation of bioactive compositions in solid state fermentation of Antrodia cinnamomea
Authors:	王皓緯 Wang, Hao-Wei
Contributors:	楊芳鏘 Yang, Fan-Chiang 東海大學化學工程與材料工程學系
Keywords:	三?;果皮;蕎麥;樟芝 citrus;buckwheat;Antrodia cinnamomea;metabolites
Date:	2011
Issue Date:	2011-10-12T07:52:35Z (UTC)
Abstract:	近年來，真菌發酵產物與其菇類子實體，其藥理與生理活性成份受到重視。其中以台灣獨有的真菌-樟芝（Antrodia cinnamomea）更是學術研究重點之一。實驗利用添加物，刺激樟芝菌絲體提升二代謝產物含量。結果發現，在添加金桔皮乾燥粉末 1 g/瓶培養 28天後分析發酵物基質(Substrate)得知粗三?含量增加 63.84% ，其次為葡萄柚皮粉末與柚子皮粉末 24.46% 與 19.47%。改變添加濃度變化得知，金桔在 2 g/瓶可以達到最高的粗三?含量 32.55 mg/g sub.。將金桔果皮經過水淬取後將水淬取液與殘渣添加進行培養後試驗，得知水淬取與淬取殘渣都可以在第28天提升葡萄糖胺含量，而金桔果皮則會低於控制組；結果顯示三種培養的粗三?含量都高於控制組，但是總多酚含量和總類黃酮含量則低於控制組。為了探討粗三?生成機制和總多酚的相關性，選取了本身?類較少而總多酚含量與柑橘果皮相同的天然物做添加，結果顯示葡萄果皮添加 1 g/瓶在第28天葡萄糖胺含量與粗三?類含量皆高於控制組而達到 3.84 mg/g sub.與 28.07 mg/g sub.。實驗結果皆證實使用天然物添加，對於樟芝菌絲體提高代謝產物是有十分明顯的影響與效果。 In recent years many medicinal mushrooms have been well-known to contain various physiological components with bioactive functions. Antrodia cinnamomea is an endemic medical mushroom in Taiwan and their major effective components are polysaccharides, steroids and triterpenoids. However, the fact that the fruiting body grow extremely slow make it difficult to be used very popularly.The aim of this research was to investigate the feasibility of enhancing the production of bioactive components by adding fruit peels into the media for solid-state fermentation(SSF) of Antrodia cinnamomea. The results show that peel powder made from kumquat, grapefruit, pemole could increase triterpenoid content in mycelia by 63.84%, 26.46% and 19.47%, respectively. In addition, crude triterpenoid concentration reaches to 32.55 mg/g sub at the concentration of 2g kumquat /peck. When different kinds of kunmquat extract(kumquat peel, water extract, kumquat pomace, control) were added and compared, the addition of water extract and kumquat pomace were found to be beneficial to the production of crude triterpenoid and glucosamine at the 28 day. In contrast kumquat peel showed some inhibitory effect on the formation of glucosamine. It is interesting to note that all kind additives had a negative effect on the production of total phenol and flavonoids. In order to investigate the biotransformation mechanism, grape and apple peel, whose fruit peels contain less amounts of terpenoids, were added into the media for SSF. The results reveal that the addition of grape peel could also enhance the formation of crude triterpenoid, which reached to 28.07 mg/g sub at the 28 day. This study demonstrates that the formation of bioactive components in the mycelia of Antrodia cinnamomea could be enhanced by adding fruit peel into the media.
Appears in Collections:	[化學工程與材料工程學系所] 碩博士論文

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