果蠅細胞移行與細胞型態發生的機制---型態發生的分子機轉---以果蠅複眼感小體發生為模式(II)

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Title:	果蠅細胞移行與細胞型態發生的機制---型態發生的分子機轉---以果蠅複眼感小體發生為模式(II)
Other Titles:	The Molecular Mechanism of Morphogenesis---Using Drosophila Rhabdomere as a Model System (II)
Authors:	范聖興 Fan, Seng-Sheen
Contributors:	行政院國家科學委員會東海大學生物系
Keywords:	複眼，感小體，型態發生，細胞極性基因 Compound eye, Rhabdomere, Morphogenesis, Cell polarity, Dmob2 gene
Date:	2004
Issue Date:	2011-11-07T12:42:38Z (UTC)
Abstract:	形態發生是發育過程中非常重要的一個步驟，它可以決定細胞、組織或是器官最後的形態。目前有關發育過程形態發生的分子調控機轉尚不清楚，本研究計畫以果蠅複眼感小體的發育為研究模式以探討基因與形態發生之調控。果蠅複眼的感小體是位於感光細胞的頂部，感小體形態發生開始於蛹期，經由細胞劇烈的形狀改變後形成了成蟲時所見的感光細胞。過去的研究顯示細胞極性基因與感小體形態發生有密切的關係。果蠅的感小體相當於脊椎動物感光細胞之外節部，脊椎動物感光細胞之外節部的發育與維持對於感光細胞之功能有密切的影響，過去研究顯示若感光細胞之外節部的發育與維持受到阻礙則會導致視覺的喪失，因此，研究果蠅感小體的形態發生亦有助於我們瞭解脊椎動物感光細胞之外節部的發育與維持。本實驗室過去主要研究微管動蛋白dynactin與細胞極性基因crumbs對感小體形態發生的作用機轉，本計畫中我們發現一個新穎的基因可能與感小體的形態發生有關，利用RNA Interference的方式抑制Dmob2 基因之表現，我們發現在感小體之發育變的不規則，而且極性蛋白(Dlt)之表現位置亦受到影響。此結果顯示Dmob2 基因可能參與細胞極性基因之決定並進而影響感小體之發育。 Morphogenesis is an important process in development to build a specific cell type, tissue or organ. Currently, the molecular regulation of the morphogeensis remains unclear. My lab uses Drosophila retina as system to study the genetic regulation of morphogenesis. Rhabdomere is a photosensitive structure localizes at the apical surface of the photoreceptor cells (PRCs). Rhabdomere morphogenesis occurs at the early pupal stage via turning and extension of the apical surface and establishment of the rhabdomere and stalk domain in the apical surface of PRCs. We have previously showed that dynactin (Glued), the dynein activator and the apical polarity gene, crumbs affect rhabdomere morphogenesis. In this study, we reported to identify a novel gene, Dmob2, plays a significant role in mediating rhabdomere morphogenesis. Using RNA interference to down-regulate the expression of Dmob2, we found the rhabdomere became irregular. In addition, the localization of apical marker, Dlt, was altered when expression of Dmob2 was down-regulated. Together, we identified a novel gene, Dmob2, in mediating rhabdomere morphogenesis.
Relation:	研究編號：NSC93-2321-B029-001 研究期間：2004-08 ~ 2005-07
Appears in Collections:	[畜產與生物科技學系所] 國科會研究報告

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