在最佳條件([S]=1%,[E]/[S]=0.2%,pH=8.0,60℃,4hr)下反應，蛋白水解液透明而有胜肽味、略帶黃色。凍乾粉末呈乳白色。1g粉末添加於100mL脫脂鮮乳中無苦味或異味，其總接受度與鮮乳無顯著差異。以濾膜分區，分子量越小的區分其IC50越低，分子量小於3000的區分其IC50為25μg/mL；以分子篩管柱(superdex peptide)可顯示不同分子量區分的分子量分佈圖。胃中pepsin對水解液之ACE抑制作用不呈破壞性，腸胃之消化作用反而略微提高水解物之ACE抑制活性。SHR動物試驗單次灌食0.186g/kg body wt.能有效降低收縮壓(約20-25mmHg)，添加phosphatidyl choline做成lipsome能提高抑制效果。 Egg white hydrolysate solution from thermolysin digestion of egg white under optimal condition was pale yellow, pepton odor and bland taste. Freeze-dried powder of hydrolysate was white in color. Taste, color and overall acceptance of skim milk containing hydrolysate powder (1g hyrolysate powder/100 ml milk) showed no significant different from regular skim milk. Different fractions from membrance separation gave different ACE inhibitory activity. The IC50 for hydrolysate with MW<3000 Da was 25 ug/ml.ACE inhibitory activity of hydrolysate was not affected by pepsin digestion and was small increased by pancreatin digestion. One single oral administration of 0.186g/kg body weight to SHR would lowed the blood pressure around 20 to 25 mmHg. Liposome treatment of hydrolysate was found to have better effectiveness in lowering blood pressure.
