幾丁聚醣水解物-三聚磷酸鈉-表沒食子兒茶素沒食子酸酯奈米顆粒之製備及其特性之研究

Tunghai University Institutional Repository > 農學院 > 食品科學系所 > 碩士論文 > Item 310901/1628

Please use this identifier to cite or link to this item: http://140.128.103.80:8080/handle/310901/1628

Title:	幾丁聚醣水解物-三聚磷酸鈉-表沒食子兒茶素沒食子酸酯奈米顆粒之製備及其特性之研究
Other Titles:	Preparation and Characterization of Chitosan Hydrolysate-Sodium Tripolyphosphate-Epigallocatechin Gallate Nanoparticles
Authors:	楊欣樺 Yang, Zin-Hua
Contributors:	阮進惠 Rwan, Jin-Hwei 東海大學食品科學系
Keywords:	表沒食子兒茶素沒食子酸酯;三聚磷酸鈉;幾丁聚醣水解物;奈米顆粒;包覆率;安定性 Entrapment;Nanoparticles;Epigallocatechin gallate (EGCG);Sodium tripolyphosphate (TPP);Chitosan hydrolysate;efficiency;stability
Date:	2008
Issue Date:	2011-03-07T03:44:52Z (UTC)
Abstract:	本實驗以纖維酵素降解幾丁聚醣製備低分子量之幾丁聚醣。選擇四種低分子量之幾丁聚醣水解物（chitosan hydrolysate，CS），分別配置不同濃度（0.67、1.00、1.34、1.68及2.00 mg/mL）及不同pH（3.0、4.7及6.0）之幾丁聚醣溶液。將上述幾丁聚醣溶液分別與不同pH（3.0、5.0及9.0）之三聚磷酸鈉（TPP）溶液（0.84 mg/mL），使其CS/TPP重量比為2/1、3/1、4/1、5/1及6/1反應，製備CS-TPP奈米顆粒及包覆不同濃度（0.1、0.2、0.3、0.4及0.5 mg/mL CS solution）之EGCG（epigallocatechin gallate）以製備幾丁聚醣-三聚磷酸鈉-EGCG（CS-TPP-EGCG）奈米顆粒，並測定EGCG之包覆率、CS-TPP及CS-TPP-EGCG奈米顆粒粒徑大小、表面電荷。掃瞄式電子顯微鏡（SEM）照相觀察，以及利用FTIR分析幾丁聚醣、TPP及EGCG之間是否產生交互作用，之後模擬CS-TPP-EGCG奈米顆粒在模擬胃液（pH 1.2）及腸液（pH 7.4）下之安定性。結果發現，在EGCG包覆率方面，以幾丁聚醣溶液 pH 4.7和TPP溶液pH 9.0反應、愈小分子量之幾丁聚醣、高CS/TPP之重量比及愈低EGCG之濃度條件下反應作用，可得較高EGCG之包覆率。在CS-TPP-EGCG顆粒粒徑方面，愈高pH之TPP溶液、CS/TPP重量比及EGCG濃度，其顆粒粒徑愈大。CS-TPP-EGCG顆粒對EGCG包覆率範圍為29-47 %。CS-TPP-EGCG顆粒粒徑均屬奈米級，範圍約為244-438 nm。此外CS-TPP-EGCG顆粒粒徑均為大於CS-TPP顆粒粒徑（226-315 nm）。在FTIR方面，幾丁聚醣和TPP產生離子交聯反應，而EGCG與幾丁聚醣或與TPP之間交互作用很小，以物理性包覆作用。CS-TPP及CS-TPP-EGCG顆粒表面均帶正電荷，兩者正電荷之差異不大，約為25.1-28.8 mV。在安定性方面，CS-TPP-EGCG奈米顆粒在模擬胃液（pH 1.2 buffer）及腸液（pH 7.4 buffer）下皆具有良好的安定性。 Low molecular weight of chitosans were prepared by cellulase hydrolysis. Four kinds of molecular weight of chitosan hydrolysates (CS) were chosen and prepared for different concentrations (0.67, 1.00, 1.34, 1.68 and 2.00 mg/mL ) and pH (3.0, 4.7 and 6.0) of CS solution. Sodium tripolyphosphate (TPP) solution ( 0.84 mg/mL ) was prepared at different pHs of 3.0, 5.0 and 9.0. CS-TPP nanoparticles were prepared by mixing the above CS and TPP solutions with different CS/TPP mass ratio 2/1-6/1. CS-TPP-EGCG nanoparticles were also prepared by adding different concentrations (0.1-0.5 mg/mL CS solution) of epigallocatechin gallate (EGCG) to the TPP solution before mixing with CS solutions. EGCG encapsulation efficiency, particles size and zeta potential of the nanoparticles were investigated. Cross linking among CS, TPP and EGCG, SEM and stabilities (at pH 1.2 and 7.4) of the nanoparticles were also determined.As result, a high encapsulation efficiency obtained in the following conditions : at pH 4.7 and pH 9.0 for the CS solution and the TPP solution, respectively, small CS Mw, high CS/TPP mass ratio and low EGCG concentration while large nanoparticle size appeared in case of small CS Mw, high CS/TPP mass ratio and high EGCG concentration. The EGCG encapsulation efficiency changed in a range of 29-47%. The nanoparticle size of CS-TPP-EGCG appeared at 244-438nm, and larger than that of CS-TPP particle (226-315nm). FTIR analysis showed that cross linking occurred between CS and TPP, but not between CS and EGCG or between TPP and EGCG. All the nanoparticles showed positive charge (+25.1-+28.8 mV). The nanoparticles were very stable in the pH 1.2 and pH 7.4 buffers for 2 hr and 6hr, respectively.
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