白藜蘆醇對3T3-L1前驅脂肪細胞分化之調控機制

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Title:	白藜蘆醇對3T3-L1前驅脂肪細胞分化之調控機制
Other Titles:	Regulatory Mechanism of the Differentiation of 3T3-L1 Preadipocytes by Resveratrol
Authors:	王茹婕 Wang, Ju-Chieh
Contributors:	張中和;盧錫祺 Chang, Chung-Ho;Lu, Hsi-Chi 東海大學食品科學系
Keywords:	白藜?醇;脂肪細胞新生作用;分化 resveratrol;adipogenesis;differentiation
Date:	2009
Issue Date:	2011-03-07T03:44:59Z (UTC)
Abstract:	白藜?醇廣泛存在於葡萄、莓果與花生等蔬果中。已知白藜?醇擁有許多生?活性，如抗氧化、抗?化和免疫調節活性等。脂肪組織可分?多種因子與細胞激素，在能?平衡上扮演重要的角色。本?文探討白藜?醇對於3T3-L1 前驅脂肪細胞分化之影響及其機制。實驗結果發現早期（day -1 到day 2）添加15μM 白藜?醇能增加末端分化指標，如GPDH活性、油脂?積與脂肪結合蛋白（aP2）和脂?素等基因表現。而PPARgamma與C/EBPalpha為脂肪分化之主要轉?因子。發現添加15μM 白藜?醇具有增加PPARgamma與C/EBPalpha之基因表現。而添加15μM 白藜?醇可增加PPARgamma、C/EBPalpha與C/EBPdelta之蛋白質表現，而些微增加C/EBPbeta之表現。此外，已知CHOP-10可與C/EBPbeta結合而抑制脂肪新生作用，結果發現添加15μM白藜?醇可?低CHOP-10之基因與蛋白質之表現。另外，我們發現ERK1/2並??與白藜?醇誘發脂肪細胞生成過程，而AKT則可能?與其中但其具體角色尚待釐清。這些結果顯示白藜?醇可藉由?低CHOP-10與增加C/EBPbeta、C/EBPdelta、C/EBPalpha和PPARgamma而促進脂肪細胞中之脂肪新生作用。 Resveratrol (trans-3,4',-5-trihydroxystilebene) is found in various vegetables and fruits, including grapes, berries and peanuts. Resveratrol exerts antioxidative, anti-aging and immunomodulatory activities. Adipose tissue plays an important role in energy homeostasis by secreting various factors and cytokines. We investigated the effect of resveratrol on 3T3-L1 preadipocyte differentiation and its underlying mechanism. Our results showed that resveratrol enhanced the induction of terminal differentiation markers such as glycerol-3-phosphate dehydrogenase (GPDH) activity, lipid accumulation, and the mRNA expression of adipocyte fatty acid-binding protein (aP2) and adiponectin when 3T3-L1 cells were treated with 15?M resveratrol during the early period (from day -1 to day 2). Peroxisome proliferator-activated receptor (PPAR)gamma and CCAATT enhancer binding proteins (C/EBP) alpha are master transcription factors of adipocyte differentiation. Resveratrol (15?M) treatment enhanced the mRNA expression of C/EBPbeta and PPARgamma which are master transcription factors of adipocyte differentiaion. 15?M Resveratrol treatment enhanced the protein expression of C/EBPdelta?C/EBPalpha and PPARgamma, and slightly enhanced the expression of the active isoform of C/EBPbeta. On the other hand, 15?M resveratrol down-regulated the mRNA and protein expression of CHOP-10, which is known to prevent adipogenesis by directly binding to C/EBPbeta. We found AKT, but not ERK1/2, maybe play a part in resveratrol-induced adipogenesis, though its role is not clear. These results suggest that resveratrol nay enhance adipogenesis of adipocytes via down-regulation of CHOP and up-regulation of C/EBPbeta, C/EBPdelta, C/EBPalpha and PPARgamma.??
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