豬皮膠原蛋白（pig skin collagen ）經三偏磷酸鈉（sodium trimetaphosphate ， STMP）予以磷酸化製成水溶性之磷酸化豬皮膠原蛋白（phosphorylated pig skin collagen， PPSC）後，續以此為基質，添加木瓜酵素（1g/100g基質）與白胺酸已 酯（leucine hexyl ester， leu-OC?腹^（0.03MOL/100基質）於pH 9，37℃下反應 30min 後，幾近全部之白胺酸己酯可被導入PPSC之α?紐儥珘搳A而製成具極優良 乳化性與起泡性之磷酸化豬皮膠原蛋白酵素修飾物（phosphorylated pig Skin collagen-leu-OC??，PPCS-leu-OC?腹^。生產此1Kg PPCS-leu-OC?萱珨搷鰹?言? 約356元，若將木瓜酵素用量減半，而白胺酸己酯減為原來2/3時，則約可降低成 本1/4，但反應時間需延長至90min。 The pig skin collagen was treated with sodium trimetaphosphate（ STMP） and then incubated with papain （ 1g/100g protein and leucine hexyl ester （ leu-OC ?腹^（ 0.03mol/100g protein ） at pH 9, 37 ℃ for 30min. Almost all of leu-OC ?? were in- corporated onto the C-terminal of α?? chain in phosphorylated pig skin collagen （ PPSC ）. The final product PPCS-leu-OC ?? had excellent functionalities and was suitable for use as a low-fat content proteinaceous surfactant. The computed cost of materials for manufacturing of one kg of PPSC-leu- OC ?? from pig skin was about NT$ 356, but if the consumptions of papain and leu-OC ?? were adjusted to half and to 2/3 of original amount, respectively, and with the increased the reaction time to 90min, the cost might reduced another 25 ％．
