本研究將鳳凰木（Delonix regia）花瓣以含酸甲醇萃取，萃取濃縮物利用 Amberlite XAD-7、Toyopearl WH-40F進行液相管柱層析後，再以高效液相層析純化獲得兩 種主要花青素；純化所得之色素利用光譜分析進行構造鑑定，得知分別為cyanidin-3-O-β -rutinoside和cyanidin-3-O-β-glucoside。同時以硫氰酸鐵法進行抗氧化性檢測，結果顯 示從鳳凰花中萃取所得這兩種花青素皆具抗氧化性，其中後者的抗氧化性與α-生育醇相同。 The acidic methanol extract of flowers of Delonix regia were separated and fractionated by Amberlite XAD-7, Toyopearl HW-40F chromatographies, then isolated by HPLC to obtain two major anthocyanins. The isolated anthocyanins were identified as cyanidin-3-O-β-rutinoside and cyanidin-3-O-β-glucoside by the instrumental analysis. The antioxidative activities for the anthocyanins were measured by the ferric thiocyanate method. The result showed that both anthocyanins had antioxidative activity. The latter anthocyanin exhibited the same activity as that of α-tocopherol.
