梅子體胚可製成人工種子，做為作物大量繁殖之用，此外體胚可用於生產種子中之 一次及二次代謝物。體胚培養可作為研究種子生理及生化現象（一次代謝或二次代謝）之重 要工具。本計畫以大青、二青及胭脂三品種之末成熟胚為試驗材料，探討植物生長調節劑、 蔗糖濃度、旋轉式懸浮培養等因子對於梅子胚直接及間接體胚發生之影響。 試驗結果發現有部分培植體可直接產生體胚。由於直接發生之體胚數量有限，因此自癒 合組織誘導間接體胚產生。癒合組織之產生以大青品種最佳，其中植物生長調節劑以NAA及 BAP組合較2，4-D及kinetin組合效果較佳。間接體胚之誘發方法，乃在繼代培養中逐漸降低 植物生長調節劑濃度至完全不添加，促進梅子間接體胚之發生，將直接或間接體胚置於適當 培養基中，可繼續繁生體胚。 The somatic embryos of mume (Prunus nume) can be used either to produce artificial seed for mass production or to produce primary and secondary metabolites. The culture of somatic embryos is a useful tool to study the physiology and biochemistry (primary and secondary metabolism) of mume seeds. The objective of this project was to using the immature zygotic embryos of Da-Ching, Erh-Ching, and Ian-Jy cultivars as explant materials to investigate the effect of plant growth regulators, concentration of sucrose and rotated suspension culture on the somatic embryogenesis of mumes. The results showed that explants could initiate somatic embryos directly. Since the quantity of these embryos were limited, the indirect somatic embryos were generated through the calli. The initiation of calli from the Da-Ching variety better with combinations NAA and BAP than 2, 4-D and kinetin. Indirect somatic embryos were obtained through the gradual lowering the concentration of plant growth regulators in the media. The direct or indirect somatic embryos could generate more somatic embryos during the culture.
