The chromosomes of the house bat Pipistrellus abramus from Taiwan were examined by conventional staining and by GTG-, and CBG-banding techniques. The diploid chromosome number (2n) and the fundamental autosomal arm number (FN) were 26 and 44, respectively. In the G-banded karyotype, large GTG-negative regions were detected in the pericentromeric regions of chromosomes 1 through 4, 10, 11 and X. All autosomes included centromeric constitutive heterochromatin. Large blocks of constitutive heterochromatin were observed after CBG-banding in the pericentromeric regions of chromosomes 1 through 4, 10, 11 and X. These findings were essentially identical to the results reported previously for P. abramus from Japan.