The conventional, G-band, and C-band karyotypes of the gray shrew Crocidura attenuata from Taiwan were investigated. The autosomes consist of 3 meta-or submetacentric, 4 subtelocentric and 12 acrocentric pairs. The X and Y chromosomes are submetacentric and acrocentric, respectively. The diploid chromosome number and fundamental number were determined to be 40 and 56, respectively. This karyotype is different from that of a Thai specimen previously recorded as C. attenuata.