Glutathione regulation in arsenic-induced porcine aortic endothelial cells

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Please use this identifier to cite or link to this item: http://140.128.103.80:8080/handle/310901/22592

Title:	Glutathione regulation in arsenic-induced porcine aortic endothelial cells
Authors:	Cheng, Y.-H.a, Ou, B.-R.bc, Cheng, L.-C.a, Lu, J.-H.a, Yeh, J.-Y.
Contributors:	Department of Animal Science and Biotechnology, Tunghai University
Keywords:	Arsenic;GCL;GGT;Glutathione turnover;Porcine endothelial cell
Date:	2008
Issue Date:	2013-05-21T09:11:34Z (UTC)
Abstract:	The objective was to investigate the regulation of glutathione (GSH) turnover in porcine aortic endothelial cells (PAECs) treated with sodium arsenite (NaAsO 2), arsenic trioxide (As 2O 3) or sodium arsenate (Na 2HAsO 4) up to 72 hr at 0, 1, 5, and 10 μM, respectively. Intracellular GSH and glutathione disulfide (GSSG) contents, as well as the activities and mRNA levels of glutamate-cysteine lyase (GCL; γ-glutamylcysteine synthetase) and γ-glutamyl transpeptidase (GGT), were examined. The trivalent arsenic compounds increased GSH and GSSG contents in PAECs. An increase in GCL activity was observed at 24 hr whereas an increase in GCL mRNA level was observed at 72 hr. The increase in GGT activity was only observed at 72 hr. In addition, a tendency of increase in GGT mRNA level was observed. Na 2HAsO 4 treatment did not affect GSH content and the turnover-related enzymes. A differential GSH modulation in PAECs by trivalent arsenic compounds was found. The regulatory mechanism responsible for the As 2O 3-induced GSH increase is related to the GSH-turnover enzymes, GCL and GGT, while that for the NaAsO 2-induced GSH increase may not be related to expression of GSH-turnover enzymes. ? 2008 Elsevier Ltd. All rights reserved.
Relation:	Toxicology in Vitro Volume 22, Issue 8, December 2008, Pages 1832-1839
Appears in Collections:	[畜產與生物科技學系所] 期刊論文

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