Expressed transcripts associated with high rates of egg production in chicken ovarian follicles

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题名:	Expressed transcripts associated with high rates of egg production in chicken ovarian follicles
作者:	Yang, K.T.ab, Lin, C.Y.a, Huang, H.L.a, Liou, J.S.a, Chien, C.Y.a, Wu, C.P.c, Huang, C.W.a, Ou, B.R.d, Chen, C.F.a, Lee, Y.P.a, Lin, E.C.e, Tang, P.C.a, Lee, W.C.f, Ding, S.T.e, Cheng, W.T.K.e, Huang, M.C.
贡献者:	Department of Animal Science and Biotechnology, Tunghai University
关键词:	cDNA microarray;Chicken;Egg production;Ovarian follicles;RT-PCR
日期:	2008
上传时间:	2013-05-21T09:11:48Z (UTC)
摘要:	The purpose of this study was to characterize differentially expressed transcripts associated with varying rates of egg production in Taiwan country chickens. Ovarian follicles were isolated from two strains of chicken which showed low (B) or high (L2) rates of egg production, then processed for RNA extraction and cDNA library construction. Three thousand and eight forty clones were randomly selected from the cDNA library and amplified by PCR, then used in microarray analysis. Differentially expressed transcripts (P<0.05, log 2?1.75) were sequenced, and aligned using GenBank. This analysis revealed 20 non-redundant sequences which corresponded to known transcripts. Eight transcripts were expressed at a higher level in ovarian tissue prepared from chicken strain B, and 12 transcripts were expressed at a higher level in L2 birds. These differential patterns of expression were confirmed by semi-quantitative RT-PCR. We show that transcripts of cyclin B2 (cycB2), ferritin heavy polypeptide 1 (FTH1), Gag-Pol polyprotein, thymosin β4 (TB4) and elongation factor 1 α1 (EEF1A1) were enriched in B strain ovarian follicles. In contrast, thioredoxin (TXN), acetyl-CoA dehydrogenase long chain (ACADL), inhibitor of growth family member 4 (ING4) and annexin II (ANXA2) were expressed in at higher levels in the L2 strain. We suggest that our approach may lead to the isolation of effective molecular markers that can be used in selection programs in Taiwan country chickens. ? 2007 Elsevier Ltd. All rights reserved.
關聯:	Molecular and Cellular Probes Volume 22, Issue 1, February 2008, Pages 47-54
显示于类别:	[畜產與生物科技學系所] 期刊論文

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