Loading...
|
Please use this identifier to cite or link to this item:
http://140.128.103.80:8080/handle/310901/22786
|
| Title: | The expression of caveolin by HDL in cholesterol-loaded aortic endothelial cells |
| Authors: | Chao, W.T., Yang, V.C. |
| Contributors: | Department of Life Science, College of Science, Tunghai University |
| Keywords: | Angiogenesis;c-Fos;c-Jun;Ets-1;Tea catechins |
| Date: | 2002 |
| Issue Date: | 2013-05-21T09:16:42Z (UTC)
|
| Abstract: | In this study, we used rat aortic endothelial cells and human umbilical vein endothelial cells growing in collagen gel as a model system to study the tea catechin, (-)-epigallocatechin (EGCG), on the differential expression of transcription factors, Ets-1, c-Fos, and c-Jun during endothelial morphogenesis in vitro. Cells growing in collagen gel from 0 to 2 h remained spherical. After 6 h, the cells became elongated and underwent morphogenesis. At 24 h, cells started to organize to form capillary-like tubular structures. At 48 h, most cells in the gel formed a network of branching and tubular structures. Immunohistochemistry and immunofluorescence microscopy showed that the reaction products of Ets-1, c-Fos, and c-Jun presented predominantly in the nucleus. No reaction products appeared in the cells that were organized to form capillary-like tubular structures. After adding EGCG to the collagen gel, cells became elongated in the first 6 h and then remained quiescent. No tubular structure was formed. Western blotting showed that the levels of Ets-1, c-Fos, and c-Jun reached the highest levels at 12-24 h, decreasing to the basal level at 48 h. After adding EGCG to the collagen gel, levels were lower than for the non-EGCG-treated groups. These results indicated that the morphogenesis of endothelial cells in collagen gel was inhibited by EGCG through the down-regulation of Ets-1, c-Fos, and c-Jun. ? 2004 Elsevier Ireland Ltd. All rights reserved. |
| Relation: | Microscopy and Microanalysis 8 (SUPPL. 2) , pp. 942-943 |
| Appears in Collections: | [生命科學系所] 期刊論文
|
Files in This Item:
| File |
Size | Format | |
|---|
| index.html | 0Kb | HTML | 150 | View/Open |
|
All items in THUIR are protected by copyright, with all rights reserved.
|
