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http://140.128.103.80:8080/handle/310901/22806
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| Title: | Novel DNA-peptide interaction networks |
| Authors: | Huang, J.T.B.a, Chen, Y.-C.a, Chang, J.-C.a, Jeng, K.-C.G.b, Kao, K.K.L.a, Yang, R.C.K.a, Kan, L.-S.cd, Wey, M.-T.c, Waring, M.J.e, Chen, C.-S.f, Chien, W.-J.f, Sheh, L.a |
| Contributors: | Department of Chemistry and Life Science Center, Tunghai Christian University |
| Keywords: | Circular dichroism;DNA-peptide molecular recognition;Footprinting;Interaction networks;Positive cooperative binding |
| Date: | 2010 |
| Issue Date: | 2013-05-21T09:17:14Z (UTC)
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| Abstract: | Allostery in the binding of peptides to DNA has been studied by quantitative DNase I footprinting using four newly designed peptides containing the XP(Hyp)RK motif and N-methylpyrrole (Py) moieties. Apparent binding constants in the micromolar range as well as Hill coefficients were determined for each peptide. The results, together with previous studies on five other peptides support the proposal that interaction network cooperativity is highly preferred in DNA-peptide interactions that involve multiple recognition sites. It is envisaged that interstrand bidentate interactions participate in the relay of conformational changes between recognition sites on the complementary strands. Models for interpreting DNA allostery based upon interaction networks are outlined. Circular dichroism experiments involving the titration of peptides against a short oligonucleotide duplex indicate that some of these peptides bind in a dimeric manner to DNA via the minor groove, inducing characteristic conformational changes. These insights should prompt the design of new DNA-binding peptides for investigating allosteric interactions between peptides and DNA, as well as novel interaction networks, and ultimately may shed light upon the fundamental chemical rules that govern allostery in more complex biological process such as DNA-protein interaction networks. ? 2010 Elsevier Ltd. All rights reserved. |
| Relation: | Bioorganic and Medicinal Chemistry
Volume 18, Issue 7, 1 April 2010, Pages 2575-2585 |
| Appears in Collections: | [化學系所] 期刊論文
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