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http://140.128.103.80:8080/handle/310901/22895
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| Title: | Influence of alanine uptake on Staphylococcus aureus surface charge and its susceptibility to two cationic antibacterial agents, nisin and low molecular weight chitosan |
| Authors: | Chen, L.-C.a , Chiang, W.-D.b , Chen, W.-C.a , Chen, H.-H.a , Huang, Y.-W.c , Chen, W.-J.d , Lin, S.-B.ad |
| Contributors: | Department of Food Science, Tunghai University |
| Keywords: | Alanine;Anionic surface charge;Cationic antibacterial agent;Low molecular weight chitosan;Nisin;Zeta potential |
| Date: | 2012 |
| Issue Date: | 2013-05-24T09:07:52Z (UTC)
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| Abstract: | Low molecular weight chitosan (LMWC) and nisin, recognized as cationic antibacterial agents (CAAs), inhibit bacterial growth by interacting with the anionically charged cell wall. In this study, alanine uptake significantly reduced the anionic cell surface charge, as determined by the zeta potential (ZP) measurements, of Staphylococcus aureus, resulting from the incorporation of d-alanine into the cell wall. Minimum inhibitory concentration (MIC) tests and growth inhibition curves revealed that LMWC and nisin possessed inverse antibacterial activity against three strains of S. aureus, depending on the strains' net charge. A twofold reduction in the MIC value of nisin was obtained against S. aureus, inoculated in a 1.0% d- or l-alanine-augmented trypticase soy broth medium. A flocculation test demonstrated that neutralizing the anionic surface charge using d-alanine reduced the adsorption of S. aureus onto LMWC. Furthermore, the reduced surface net charge could enhance the colonization capacity of S. aureus on glass. ? 2012 Elsevier Ltd. All rights reserved. |
| Relation: | Food Chemistry
Volume 135, Issue 4, 15 December 2012, Pages 2397-2403 |
| Appears in Collections: | [食品科學系所] 期刊論文
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