Sonic Hedgehog improves in vitro development of porcine parthenotes and handmade cloned embryos

Tunghai University Institutional Repository > 農學院 > 畜產與生物科技學系所 > 期刊論文 > Item 310901/23136

Please use this identifier to cite or link to this item: http://140.128.103.80:8080/handle/310901/23136

Title:	Sonic Hedgehog improves in vitro development of porcine parthenotes and handmade cloned embryos
Authors:	Nguyen, N.T., Lin, D.P.C., Siriboon, C., Lo, N.-W., Ju, J.-C.
Contributors:	Department of Animal Science and Biotechnology, Tunghai University
Keywords:	Apoptosis;Comet assay;Embryo development;Parthenogenesis;Shh
Date:	2010
Issue Date:	2013-06-11T09:01:07Z (UTC)
Abstract:	This study investigated the expression of Sonic Hedgehog (Shh) signaling pathway and its effect on porcine parthenogenetic (PA) embryo development. The Shh receptor Patched (Ptc1) and co-receptor Smoothened (Smo) were expressed at various stages of PA porcine embryos, at both mRNA and protein levels. Furthermore, the transcriptional activator Gli1 mRNA was first present in the 2-cell stage embryos, and was readily detected at the 4-cell stage and beyond. Culture medium supplemented with 0.5 μg/mL Shh optimized blastocyst rates (58.6 vs. 41.1%; P < 0.05) and the total number of cells per blastocyst (56.4 vs. 45.6 cells; P < 0.05); however, this response was prevented by simultaneous addition of 1 mM cyclopamine (an Shh inhibitor). Moreover, blastocysts that developed in medium containing 0.5 μg/mL Shh had lower apoptotic indices and reduced DNA damage (evaluated by TUNEL and comet assays, respectively). Based on Western-blot analysis, expression of phosphorylated Akt protein in Shh-treated blastocysts was higher than that of the control group (1.22- vs. 0.66-fold, P < 0.05), and less total PARP-1/2 protein was accumulated (0.7-fold, P < 0.05) in treated blastocysts compared to untreated controls. Furthermore, supplementation of Shh (1 μg/mL) also supported development of handmade cloned embryos (50.3 vs. 26.8%; P < 0.05) with reduced apoptotic rates (2.8 vs. 6.3%; P < 0.05). We inferred that the Shh signaling pathway existed in porcine PA embryos and we concluded that Shh supplementation improved the quality and developmental competence of early PA embryos, at least in part, by increasing cell proliferation and reducing apoptosis of the developing embryos. ? 2010 Elsevier Inc.
Relation:	Theriogenology 74 (7) , pp. 1149-1160
Appears in Collections:	[畜產與生物科技學系所] 期刊論文

Files in This Item:

File	Size	Format
index.html	0Kb	HTML	220	View/Open

Loading...