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http://140.128.103.80:8080/handle/310901/23381
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| Title: | Taiwanese Journal of Agricultural Chemistry and Food Science |
| Authors: | Ho J.-H., Chow C.-F., Su H.-P., Lee T.-A. |
| Contributors: | Department of Nutritional Science, Toko University, No. 51, Hsueh Fu Rd., Pu-Tzu, Chia-Yi, 61363, Taiwan;Department of Animal Science and Biotechnology, Tunghai University, No.181, Taichung Harbor Rd., Situn District, Taichung, 40704, Taiwan;Department of Animal Science and Technology, National Taiwan University, No.50, Ln.155, Keelung Rd., Taipei, 10617, Taiwan;Kunshan Fusheng Food Development, Co., Ltd., 4F-8, No.9, Donghan St. North Dist., Taichung, 40465, Taiwan |
| Date: | 2010 |
| Issue Date: | 2013-06-24T09:04:04Z (UTC)
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| Abstract: | ε-Polylysine-dextran conjugate is a compound conjugated with ε-polylysine (PL) and dextran through the Maillard reaction. The antimicrobial activity of PL-dextran conjugate was investigated using six Grampositive and Gram-negative bacteria. The integrity of the cell membranes of the representative bacteria was determined by the amount of intracellular components released from the cells. It was found that the PL-dextran conjugate retained most of the original antimicrobial activity of PL. When treated with PL or the PL-dextran conjugate, the amount of releasing materials at absorbance of 260 nm quickly increased in both Gram-negative and positive bacteria, but the absorbance values were different due to the difference in cell structures. Furthermore, an outer membrane (OM) permeability assay was performed by fuorescent probe 1-N-phenylnaphthylamine (NPN). The results showed that PL or PL-dextran conjugate rapidly increased the NPN uptake. In addition, the preservative effect and the emulsifying properties of the PL-dextran conjugate were also observed when it is applied in coffee whitner. These fndings indicate that the PL-dextran conjugate can be applied in the food processing industry. |
| Relation: | Antibacterial mechanism and application of ε-polylysine conjugation with dextran 48(6) |
| Appears in Collections: | [畜產與生物科技學系所] 期刊論文
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