細胞移行是一個很重要的生物功能，其與胚胎發育、器官形成、傷口癒合、免疫反應與癌症轉移都有關係。癌症轉移是造成癌症死亡的主要原因，因此，瞭解細胞移行的分子機制可以提供我們尋找預防癌症轉移的線索。MOB2蛋白已知可以去調控酵母菌的細胞極性與細胞型態；在果蠅中MOB2則可以影響翅膀型態與感光細胞型態；在哺乳類MOB2與中心粒的分裂和細胞死亡訊息有關連。在先前的研究中，我們發現MOB2蛋白可誘發老鼠N2A細胞的神經纖維形成；也能影響人類肝癌細胞株Mahlavu細胞的細胞移行。此次的實驗裡，我們利用LS-SCA5組織晶片，來評估hMOB2在人體組織的表現。透過免疫組織化學染色，我們發現hMOB2在人體各個組織都有不等程度的表現，而在癌症組織中hMOB2的表現比正常組織來更高，代表著hMOB2與癌症的形成與惡化可能有關係。為了評估hMOB2的細胞功能為何?我們利用大腸癌細胞株HT29細胞來進行體外實驗。我們發現hMOB2主要分布於HT29細胞的細胞質。當HT29細胞的hMOB2表現被RNA干擾抑制時，HT29細胞的增生能力與移行能力都有顯著的受到抑制。這說明著hMOB2蛋白與細胞增生與移行有關。綜合以上發現，我們認為hMOB2蛋白與癌症的發生與惡化具有重要的角色，值得未來進一步的研究。 Cell migration is an important biologic function. It participates in several biologic processes, such as embryo development, organization, wound healing, immune reaction and metastasis. Metastasis is the main cause of cancer mortality. Thus, understanding the molecular regulation of cell migration may help to provide a clue to prevent cancer metastasis. It has been known that MOB2 proteins regulate cell polarity and cell morphology in yeasts, the morphology of the wing and photoreceptor cell in Drosophila, centrosome duplication and cell death signaling in mammals. In the previous study, we found MOB2 protein can induce the neurite formation in mouse N2A cell line and affect the migration of human hepatocarcinoma cell line (Mahluva cell). In this study, we used LS-SCA5 tissue microassay to evaluate the expression of hMOB2. Using immunohistochemistry stain, hMOB2 was generally expressed in varies type of tissues with different extent. Besides, we found the expression of hMOB2 is significantly increased in malignant tissues than normal tissues. This indicates hMOB2 may have a role in cancer development and progression. We further evaluated the function of hMOB2 by an in vitro assay using the colonal cancer cells (HT29 cell). We found that hMOB2 expressed in the cytoplasm of HT29 cells. When the expression of hMOB2 in HT29 cells was knocked down by shRNAi, cell proliferation and cell migration were both decreased significantly. Thus, hMOB2 protein had a role in cell proliferation and cell migration. All together, hMOB2 is a potential candidate for future study in cancer development and progression.
