本研究利用固定化Laccase來降解水中新興汙染物。固定化Laccase 之顆粒實驗結果顯示，有添加幾丁聚醣，包覆率皆能達到100 %。觀察到第十四天，Laccase活性洩漏最多只有12 %。固定化Laccase批次降解實驗結果顯示，編號BCRC35318 Laccase (BCRC) 對於IBU、NAP及Indigo降解效果不佳。而Trametes versicolor (TVER) 可在添加mediator後氧化31 % 之IBU。TVER可在第60小時氧化15 % 之NAP，添加2,2’-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS) mediator後，於第84小時可完全氧化NAP。TVER對於Indigo反應也頗差，而BCRC和TVER Laccase均能在一分鐘內完全氧化DMP。將固定化Laccase置於管柱其實驗結果顯示，BCRC Laccase對於IBU及NAP降解效果均不佳。TVER只能在添加mediator後才能氧化13 % 之IBU。TVER添加mediator後，第84小時可完全氧化NAP，其結果極佳。而BCRC Laccase能在12小時內完全氧化DMP，TVER可在半小時去除99 % 。管柱連續降解實驗中，TVER可連續降解8個批次的DMP。本研究結果顯示，固定化Laccase具有被利用的潛力，此方法預估可應用於污水處理廠之水再生處理單元中。 The objective of this study is to use immobilized Laccase beads made from calcium alginate to degrade emerging contaminants in water, including ibuprofen, naproxen, indigo, and dimethylphenol. Results indicate that beads having the lowest Laccase leakage rate were observed when made from 2 % of alginate together with 3 % calcium chloride. In batch experiments, the BCRC Laccase was found to be ineffective in degrading ibuprofen, naproxen, and indigo. However, with the presence of ABTS mediator the commercial TVER Laccase was found to be effective in degrading ibuprofen and naproxen. A complete removal of naproxen was even observed. With respect to the degradation of dimethylphenol, both BCRC and TVER Laccases can achieve complete removals within one minute. In column experiments, the BCRC Laccase is still ineffective in degrading both ibuprofen and naproxen. On the other hand, the TVER Laccase is able to degrade 13% of ibuprofen and nearly 100% of naproxen with the addition of ABTS mediator. Finally, the TVER beads were tested for reusability. It was found that TVER beads can be reused for at least 8 times in the degradation of dimethylphenol. Results from this study suggest that alginate-immobolized beads have the potential to be used in water reclamation process against emerging contaminants.
