Dark fermentative hydrogen production with crude glycerol from biodiesel industry using indigenous hydrogen-producing bacteria

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Title:	Dark fermentative hydrogen production with crude glycerol from biodiesel industry using indigenous hydrogen-producing bacteria
Authors:	Lo, Y.-C.;Chen, X.-J.;Huang, C.-Y.;Yuan, Y.-J.;Chang, J.-S.
Contributors:	Department of Environmental Science and Engineering, Tunghai University
Keywords:	Bio-hydrogen;Bio-hydrogen production;Clostridium butyricum;Clostridium pasteurianum;Dark fermentative hydrogen production;Fermentative hydrogen production;Hydrogen producing bacteria;Klebsiella sp;Biodiesel;Carbon;Clostridium;Hydrogen production;Synthetic fuels;Glycerol
Date:	2013
Issue Date:	2014-06-20T02:20:17Z (UTC)
Abstract:	Glycerol is an inevitable by-product from biodiesel synthesis process and could be a promising feedstock for fermentative hydrogen production. In this study, the feasibility of using crude glycerol from biodiesel industry for biohydrogen production was evaluated using seven isolated hydrogen-producing bacterial strains (Clostridium butyricum, Clostridium pasteurianum, and Klebsiella sp.). Among the strains examined, C. pasteurianum CH4 exhibited the best biohydrogen-producing performance under the optimal conditions of: temperature, 35 C; initial pH, 7.0; agitation rate, 200 rpm; glycerol concentration, 10 g/l. When using pure glycerol as carbon source for continuous hydrogen fermentation, the average H2 production rate and H 2 yield were 103.1 ? 8.1 ml/h/l and 0.50 ? 0.02 mol H2/mol glycerol, respectively. In contrast, when using crude glycerol as the carbon source, the H2 production rate and H2 yield was improved to 166.0 ? 8.7 ml/h/l and 0.77 ? 0.05 mol H 2/mol glycerol, respectively. This work demonstrated the high potential of using biodiesel by-product, glycerol, for cost-effective biohydrogen production. ? 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
Relation:	International Journal of Hydrogen Energy,V.38,Issue35,P.15815-15822
Appears in Collections:	[環境科學與工程學系所] 會議論文

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