探討兩階段固態培養對於紅麴菌之活性成分影響

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题名:	探討兩階段固態培養對於紅麴菌之活性成分影響
其它题名:	Two Stages Solid-state Fermentation of Monascus purpureus to Enhance the Formation of Bioactive Compounds
作者:	黃聖文 Sheng-Wun Huang
贡献者:	楊芳鏘 Fan-Chiang Yang 化學工程與材料工程學系
关键词:	兩階段培養;固態培養;紅麴菌 solid-state culture;monacolin K;Monascus purpureus;two-stage culture
日期:	2014
上传时间:	2015-05-07T01:55:29Z (UTC)
摘要:	紅麴菌（Monascus purpureus）是中國傳統的釀造菌株，一直受到相當的重視與利用，可生產色素、香氣及多種食品添加物。近年來研究發現其二級代謝產物中的 monacolin K、citrinin 及抗氧化物等。本實驗以固態培養，利用水含量50％糙米當作基質於30℃下進行培養。以不同添加物、培養方法及培養溫度來提高紅麴菌 monacolin K 含量。結果顯示，於培養第7天添加1％果糖，培養14天後分析發酵產物得知 monacolin K 含量456.7 mg/kg，為控制組的2.8倍，為初始添加的3.7倍。利用兩階段培養有助於紅麴菌生成 monacolin K。將果糖及蛋白?以濃度比5：1混合於第7天添加，可達到最高 monacolin K 含量512.1 mg/kg。為了探討溫度改變對紅麴菌代謝產物的影響，培養前7天以30℃培養，第7天後改變溫度。結果顯示，於第7天改變溫度至25℃，monacolin K 含量680.1 mg/kg，為控制組的1.2倍。實驗結果顯示第二階段溫度降低，對於紅麴菌提高monacolin K 含量有較佳的效果。最後實驗於培養第7天加入果糖與蛋白?濃度比5：1、改變溫度至25℃及以不同強度與波長光源照射樣品。結果顯示，第二階段照射 1500 lux 紅光 monacolin K 含量有最高值 790.6 mg/kg，為控制組的1.21倍。實驗結果顯示紅光有助於 monacolin K 生成。 Monascus (Monascus purpureus) is a traditional Chinese medicinal fungus and has received considerable attention for the production of some secondary metabolites such as: monacolin K, pigments, citrinin, pigments, aroma and antioxidants. Recent studies have found that the secondary metabolites mainly included monacolin K, citrinin and antioxidants.In this study, brown rice with water content of 50% was used as a basal medium in solid state cultures using 250ml flasks at 30℃ for two weeks. With different additives, the strategy of two- stage culture has been tested to improve the production of monacolin K. The results show that when fructose of 1% was added the in culture day 7, the content of monacolin K rose to 456.7 mg/kg, and was 2.8 times more than the control. If the additive was mixed by fructose and peptone with ratio of 5:1, the content of monacolin K could reach to 512.1 mg/kg. Two-stage cultures have been proved to be beneficial to the production of monacolin K.. The effects of temperature shift from 30℃ to 25℃ or 35℃ in one week was studied in two-stage cultures. The results indicate that the shift to lower temperature 25℃could enhance the content of monacolin K to 680.1 mg/kg and would be favorable to the formation on Monascus metabolites. The finally study in culture day 7 added fructose and peptone with ratio of 5:1, change the temperature to 25℃ and with different illumination and wavelength of the light source illuminating the sample. The results indicate that the illumination 1500 lux red could enhance the content of monacolin K to 790.6 mg/kg and would be favorable to the formation on Monascus metabolites
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