本實驗嘗試將表面增顯拉曼作為實際應用為研究目的。由於目前在於醫療檢測、農產品檢測以及環境檢測方面對不同種類檢體的之各項生化檢測工作的需求越來越多，但往往限制於儀器、技術及試劑消耗量等問題，傳統分析法已無法解決在於微奈米尺度下的檢測。利用本實驗樹狀金電極作為電極，在表面增顯拉曼上有著巨大的功效，其奈米金與生物低毒性，而且此電極製程簡單，成本低廉，將其朝向發展醫療檢測或生物檢測相當合適。此次研究探討結合對4-Mercaptobenzoic acid（4-MBA）以及4-Mercaptopyridine（4-MPy）其光學活性對於環境酸鹼值的靈敏反應，偵測迅速，以及顯著的拉曼訊號，再配合樹狀金電極，作為全光學的微奈米尺度下的酸鹼偵測器。為將來應用在生醫檢測上。 We researched on Raman Enhanced Scattering and tried to apply this theory into practical use. Due to more and more needs on bio-detection in many aspects such as medical, farm production, and environmental test, precisely sensing methods becomes much more important than ever. However, these kinds of detection were so restricted by equipments and technologies before that conventional analysis were useless in nano-scaling generation. By applying gold dendritic substrate, It’s has great improvement could be achieved on surface-enhanced Raman scattering (SERS).We successfully developed an optical sub-micron pH sensor basedon advantages of optical activity of 4-mercaptobenzoic acid （4-MBA）and 4- Mercaptopyridine（4-MPy） in environmental pH value such as precise sensitivity, rapid response, and obvious Raman signal. And this study will play as an fundamental role of biological chips in the future.
