The absence of ion-regulatory suppression in the gills of the aquatic air-breathing fish Trichogaster lalius during oxygen stress

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Title:	The absence of ion-regulatory suppression in the gills of the aquatic air-breathing fish Trichogaster lalius during oxygen stress
Authors:	林惠真 Lin, Hui-Chen Huang, Chun-Yen Lin, Hsueh-Hsi Lin, Cheng-Huang
Contributors:	Department of Life Science, Tunghai University Department of Chemistry, National Taiwan Normal University Center for Tropical Ecology and Biodiversity, Tunghai University
Keywords:	Air-breathing behavior Antioxidant enzyme Glycogen utilization Hypoxia Na+/K+-ATPase Labyrinth organ
Date:	2015-01
Issue Date:	2016-08-08T07:16:01Z (UTC)
Publisher:	荷蘭：Elsevier
Abstract:	The strategy for most teleost to survive in hypoxic or anoxic conditions is to conserve energy expenditure, which can be achieved by suppressing energy-consuming activities such as ion regulation. However, an air-breathing fish can cope with hypoxic stress using a similar adjustment or by enhancing gas exchange ability, both behav- iorally and physiologically. This study examined Trichogaster lalius, an air-breathing fish without apparent gill modification, for their gill ion-regulatory abilities and glycogen utilization under a hypoxic treatment. We recorded air-breathing frequency, branchial morphology, and the expression of ion-regulatory proteins (Na+/ K+-ATPase and vacuolar-type H+-ATPase) in the 1st and 4th gills and labyrinth organ (LO), and the expression of glycogen utilization (GP, glycogen phosphorylase protein expression and glycogen content) and other protein responses (catalase, CAT; carbonic anhydrase II, CAII; heat shock protein 70, HSP70; hypoxia-inducible factor-1α, HIF-1α; proliferating cell nuclear antigen, PCNA; superoxidase dismutase, SOD) in the gills of T. lalius after 3 days in hypoxic and restricted conditions. No morphological modification of the 1st and 4th gills was observed. The air- breathing behavior of the fish and CAII protein expression both increased under hypoxia. Ion-regulatory abilities were not suppressed in the hypoxic or restricted groups, but glycogen utilization was enhanced within the groups. The expression of HIF-1α, HSP70 and PCNA did not vary among the treatments. Regarding the antioxi- dant system, decreased CAT enzyme activity was observed among the groups. In conclusion, during hypoxic stress, T. lalius did not significantly reduce energy consumption but enhanced gas exchange ability and glycogen expenditure.
Relation:	Comparative Biochemistry and Physiology, Part A, 179, 7-16
Appears in Collections:	[生命科學系所] 期刊論文

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