Identification of IgE-binding epitopes and recombinant IgE reactivities of a latex cross-reacting Indian jujube Ziz m 1 allergen.

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题名:	Identification of IgE-binding epitopes and recombinant IgE reactivities of a latex cross-reacting Indian jujube Ziz m 1 allergen.
作者:	黃光裕 Lee, M F Tsai, J J Hwang, G Y Lin, S J Chen, Y H
贡献者:	Taichung Veterans General Hospital Department of Life Science, Tunghai University Chung Shan Medical University National Yang-Ming University
关键词:	IgE-binding regions Indian jujube latex–fruit syndrome recom-binant Zizm1allergen synthetic peptides
日期:	2008-06
上传时间:	2016-08-17
出版者:	USA:Wiley-Blackwell
摘要:	Zizm1isamajor Indian jujube (Zizyphus mauritiana)allergeninvolvedinlatex-fruit syndrome, and cDNA of the allergen has been cloned, sequencedand expressed in yeast by our laboratory pre viously. In this study, we per-formed an immunoglobulin E (IgE)-binding epitope analysis of Ziz m 1 usingoverlapping recombinant fragments. Eight overlapping recombinant frag-ments were generated from the recombinant Ziz m 1 allergen. The fragmentswere expressed in Escherichia coli and IgE-binding activities were evaluatedby sera of latex–Indian jujube-allergic subjects and normal subjects usingimmunoblotting. Human allergic sera are not able to recognize frag mentsconsisting of amino acid sequences 26–71, 119–280 and 119–291. However,residues at positions 26–199, 26–105, 26–86, 119–320 and 238–330 werefound relevant in the IgE-binding. Our results indicate that72NISGHCSDCTFLGEE86and292VWNRYYDLKTNYSSSIILEYVNSGTKYLP320ofZizm1are the sequences required for human IgE binding. Four correspondingpeptides,72NISGHCSDCTE86,292VWNRYYDLKT301,300KTNYSSSIILEY311and309LEYVNSGTKYLP320, were synthesized, and these peptides reacted with70%, 100%, 70% and 70% of 10 allergic sera tested, as revealed by enzyme-linked immunosorbent assay. Sensitization to292VWNRYYDLKT301correlatedsignificantly with the presence of allergic symptoms (P < 0·001). These find-ings will be useful in designing diagnostic and therapeutic approaches,thereby contributing to the development of specific immunotherapy for sub-jects with latex–fruit syndrome.
關聯:	Clinical and Experimental Immunology, 152(3), 464-471
显示于类别:	[生命科學系所] 期刊論文

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