近年來由於奈米科技蓬勃發展，奈米粒子對於環境及生物的影響也更受人矚目，因此我們有必要開發一些方法來偵測環境中的奈米粒子，其中金奈米粒子的應用也是較為廣泛的一種。本實驗則是利用金奈米粒子本身具有雷利散射（rayleigh scattering）訊號作為偵測，並以物鏡式暗視野之裝置結合毛細管電泳，來偵測金奈米粒子。學生所使用之毛細管為方形毛細管，並非傳統圓形毛細管，藉由方形毛細管的特性能夠有效降低不必要的背景散射光。分離條件上，學生選擇在毛細管內通入50 mM Glycine，為了增加其解析度，學生在近樣前端添加十二烷基硫酸鈉（sodium dodecyl sulfate, SDS），經過優化後之裝置及實驗條件下，能成功偵測到金奈米粒子之散射光，為了達到更好的偵測極限，學生運用了樣品堆積，使此系統能夠偵測到10 pM的金奈米粒子訊號。學生更近一步在無電滲流之條件下，分離電壓- 5 Kv，並藉由1% PVP做為篩分介質，且將修飾於金奈米表面之MPA替換為MUA，在此條件下，學生能夠成功的將13 nm 及32 nm之金奈米粒子從毛細管電泳中分離開來，並利用標準添加法來確定其訊號位置，亦能夠成功確認兩種不同大小之金奈米粒子。因此，學生所開發出此套系統，不僅有好的偵測極限，並且還能夠成功的將13 nm 及32 nm 之金奈米粒子分離開來。 In recent years, due to the vigorous development of nanotechnology, the impact of nanoparticles on the environment and the biology attracted much more attention. Therefore, we need to develop some methods to detect nanoparticles in the environment, which the application of gold nanoparticles is more widely used. In this study, we take advantage of gold particles which themselves have a Rayleigh scattering characteristic as a detection signal, and also collect the signal of gold nanoparticles by capillary electrophoresis with objective-type dark field scattering. We choose the square capillary instead of circular capillary. With the square capillary’s unique characteristics can effectively reduce unwanted background scattering. The separation condition of gold nanoparticles is 50 mM Glycine and in order to improve the separation efficiency, we add sodium dodecyl sulfate in the capillary. Under experimental conditions and apparatus through optimized, it can successfully detect the gold nanoparticle’s scattering. In order to have better detection limit, we use the on line sample stacking. The limits of detection of gold nanoparticles were 10 pM. We investigate in non- electroosmotic flow condition and separation voltage is -5 kV. We also modify the gold nanoparticles by MUA. Under this condition, we can separate 13 nm and 32 nm successfully by capillary electrophoresis. We even use the standard addition method to determine exactly peak location. Therefore, we develop this set of system not only have good detect limits but also can separate the different size of gold nanoparticles.
