Development and fibronectin signaling requirements of the zebrafish interrenal vessel

THUIR > College of Science > Department of Life Sciences > Periodical Articles > Item 310901/28684

Please use this identifier to cite or link to this item: http://140.128.103.80:8080/handle/310901/28684

Title:	Development and fibronectin signaling requirements of the zebrafish interrenal vessel
Authors:	劉薏雯 Chiu, Chih-Hao Chou, Chih-Wei Takada, Shinji Liu, Yi-Wen
Contributors:	Department of Life Science, Tunghai University
Keywords:	Zebrafish interrenal vessel
Date:	2012-08
Issue Date:	2016-10-26T03:09:49Z (UTC)
Publisher:	US:PLOS
Abstract:	Background The early morphogenetic steps of zebrafish interrenal tissue, the teleostean counterpart of the mammalian adrenal gland, are modulated by the peri-interrenal angioblasts and blood vessels. While an organized distribution of intra-adrenal vessels and extracellular matrix is essential for the fetal adrenal cortex remodeling, whether and how an intra-interrenal buildup of vasculature and extracellular matrix forms and functions during interrenal organogenesis in teleosts remains unclear. Methodology and Principal Findings We characterized the process of interrenal gland vascularization by identifying the interrenal vessel (IRV); which develops from the axial artery through angiogenesis and is associated with highly enriched Fibronectin (Fn) accumulation at its microenvironment. The loss of Fn1 by either antisense morpholino (MO) knockdown or genetic mutation inhibited endothelial invasion and migration of the steroidogenic tissue. The accumulation of peri-IRV Fn requires Integrin α5 (Itga5), with its knockdown leading to interrenal and IRV morphologies phenocopying those in the fn1 morphant and mutant. fn1b, another known fn gene in zebrafish, is however not involved in the IRV formation. The distribution pattern of peri-IRV Fn could be modulated by the blood flow, while a lack of which altered angiogenic direction of the IRV as well as its ability to integrate with the steroidogenic tissue. The administration of Fn antagonist through microangiography exerted reducing effects on both interrenal vessel angiogenesis and steroidogenic cell migration. Conclusions and Significance This work is the first to identify the zebrafish IRV and to characterize how its integration into the developing interrenal gland requires the Fn-enriched microenvironment, which leads to the possibility of using the IRV formation as a platform for exploring organ-specific angiogenesis. In the context of other developmental endocrinology studies, our results indicate a highly dynamic interrenal-vessel interaction immediately before the onset of stress response in the zebrafish embryo.
Relation:	PLoS One, 7(8), e43040
Appears in Collections:	[Department of Life Sciences ] Periodical Articles

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	139	View/Open

View Licence

Loading...