富含共軛亞麻油酸之優酪乳對卵白蛋白誘導之呼吸道過度反應的調節作用

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Title:	富含共軛亞麻油酸之優酪乳對卵白蛋白誘導之呼吸道過度反應的調節作用
Other Titles:	The modulatory effects of CLA-enriched yoghurt in OVA-induced airway hyperresponsiveness
Authors:	謝漢儀 Hsieh, Han-Yi
Contributors:	謝長奇 Hsieh, Chang-Chi 東海大學畜產與生物科技學系
Keywords:	過敏、抗發炎、氣喘、共軛亞麻油酸、優酪乳 Allergy, Anti-inflammation, Asthma, Conjugated linoleic acid, Yoghurt
Date:	2010
Issue Date:	2010-12-30T06:37:26Z (UTC)
Abstract:	共軛亞麻油酸在花生四烯酸的脂質代謝上扮演著重要角色，可調節發炎反應，在飲食中藉由提升共軛亞麻油酸含量的方法可緩解過敏原所誘導之過敏性氣喘，而富含共軛亞麻油酸之牛乳可降低小鼠之呼吸道過敏，但其作用機制不明，利用乳酸菌調節過敏性氣喘之作用有限。本實驗探討優酪乳或富含共軛亞麻油酸之優酪乳對卵白蛋白(OVA)誘發BALB/c小鼠之呼吸道過度反應之免疫調節機轉，透過分析脾臟組織與肺沖液中白血球之表面抗原(包括CD3、CD4、CD8、CD19、CD25、CD45、CD69、CD278、Tim-3與PanNK)以瞭解T4、T8、T、B、T調節、T毒殺、NKT、Th1、Th2、Tr1與 Tr2等細胞之分化；另一方面利用身體體積偵測器量測呼吸道過度反應；由血液與肺沖液中過敏原特異性抗體的含量及肺沖液中細胞激素的變化來評估免疫調節的交互作用；最後以蘇木紫與伊紅(hematoxylin & eosin)及甲苯胺藍(toluidine blue)染色進行組織病理判讀，評估肺部發炎及肥大細胞浸潤程度。結果顯示優酪乳與富含共軛亞麻油酸之優酪乳可以恢復T調節型細胞，它的意義在於增加抑制型T細胞，負調節Th2 (CD278+, CD4+)與提升Th1 (Tim-3+, CD4+)的表現量。另一方面，共軛亞麻油酸可以顯著性地抑制過敏性抗體IgE與發炎細胞激素IL-6的產生及肥大細胞的脫顆粒現象，進一步降低呼吸道過度反應。綜上所述，添加共軛亞麻油酸的優酪乳可顯著性地透過調節T調節細胞與Th1/Th2細胞的分化，進一步降低過敏性IgE抗體分泌與發炎現象並緩和呼吸道過度反應。 Conjugated linoleic acid (CLA) plays an important role in regulation of arachidonic acid metabolism. The way to raise the CLA content in the diet can reduce the allergen induced atopic asthma. CLA and lactic acid bacteria can modulate inflammation in bronchiolar hyperresponsiveness. The previous studies indicated that CLA-enriched milk can attenuate allergic airway disease in mice, but the mechanism is unclear. In this study, we administered yoghurt with or without various concentrations of CLA to determine their effects on OVA-induced airway hyperresponsiveness in BALB/c mice. Leukocytes harvest from bronchoalveolar lavage fluid (BALF), spleen, airway-draining and mesenteric lymph node were measurement. Cluster of differentiation markers including CD3, CD4, CD8, CD19, CD25, CD45, CD69, CD278, Tim-3 and PanNK, were used to distinguish the cells differentiation. The enhanced pause (Penh) was measured with whole body plethysmograpy to monitor the airway hyperresponsiveness. Specific antibodies including anti-OVA antibodies and cytokines in serum and BALF were measured with ELISA. The H&E and toluene blue stain of lung inflammation and mast cell infiltration were determined by microscopy. The results indicated that yoghurt and CLA enrichment restore the regulatory T cells (Treg) that increased the suppressor T cells in modulated immune response. Yoghurt and CLA could reduce the Th2 (CD278+, CD4+) and increase the Th1 (Tim-3+, CD4+) to modulate T helper cell differentiation. Another results indicated that CLA-enriched yoghurt significantly inhibited allergen-specific IgE production and reduced IL-6 in BALF. Mast cell infiltration was also reduced and decrease airway hyperresponsiveness in allergic BALB/c mice. In conclusion, CLA-enriched yoghurt is an idea healthy daily supplement for airway hypersensitivity via modulating Treg, Th1/Th2 differentiation and allergic IgE.
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