檸檬馬鞭草及鐵馬鞭草水萃取物抗氧化及醣解酵素抑制活性成分之分析比較

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题名:	檸檬馬鞭草及鐵馬鞭草水萃取物抗氧化及醣解酵素抑制活性成分之分析比較
其它题名:	Comparison on antioxidative and glycosidase inhibitory components from water extracts of Aloysia triphylla and Verbena officinalis
作者:	王仁宜 WANG,REN-YI
贡献者:	蘇正德 SU,JENG-DE 食品科學系
关键词:	檸檬馬鞭草 Aloysia triphylla
日期:	2018
上传时间:	2018-03-30T01:29:20Z (UTC)
摘要:	糖尿病(Diabetes mellitus；DM)與胰島素分泌不足或作用缺失相關，其形成的高血糖使體內產生過多活性氧，造成氧化壓力上升及相關併發症發生，所以抗氧化物質對於活性氧所引發疾病之調控中扮演重要角色。而醣解酵素不但參與人體消化作用，也影響如糖尿病的許多代謝疾病產生，醣解酵素中以α-澱粉酶(α-amylase)及α-葡萄醣苷酶(α-glucosidase)最為重要。中藥草馬鞭草(Verbena officinalis L.)又稱鐵馬鞭，已證實其具有抗氧化、抗發炎、保護神經、止痛、抗真菌等生理功能，本研究室曾自鐵馬鞭草之正丁醇萃取物分離出具抗氧化及抑制醣解酵素作用之isoverbascoside (1)、verbascoside (2)、eukovoside (3) 、eukovoside isomer (4) 及β-hydroxyverbascoside (5)等成分，但鐵馬鞭草並非藥食兩用植物，較不利於發展保健食品；本研究主旨是利用鐵馬鞭草(V. officinalis L.)與藥食兩用之檸檬馬鞭草(Aloysia triphylla)為實驗材料，分析比較其抗氧化性及醣解酵素抑制活性成分。二種馬鞭草分別經由熱水萃取(2kg乾草/100L水)及超音波震盪萃取30min重複3次，將得到之粗萃物進行XAD-7、ODS管柱層析，再經由HPLC分析與製備，最後經由LC/MS/MS及1H-NMR等光譜進行抗氧化及醣解酵素抑制活性成分之解析及比較。結果顯示，檸檬馬鞭草粗萃物之抗氧化活性總酚、總類黃酮含量，總抗氧化、DPPH抑制及抗醣解酵素α-glucosidase抑制活性顯著高於鐵馬鞭草，α-amylase抑制活性則沒有顯著差異。在波長280nm之下，XAD-7管柱層析區分物，鐵馬鞭草區分出I~V個區分物，檸檬馬鞭草區分出I~VI個區分物，而在鐵馬鞭草第III區分物與檸檬馬鞭草第IV區分物中，其抗氧化活性及抗醣解酵素皆顯著高於其他區分物；檸檬馬鞭草第IV區分物其抗氧化活性總酚、總類黃酮、總抗氧化能力、DPPH抑制率及α-glucosidase抑制活性皆顯著高於鐵馬鞭草第III區分物，α-amylase抑制活性則沒有顯著差異。上述區分並利用ODS管柱層析進行進一步分離，目前得知活性區分都落在第I及第II區分上；並繼續經由HPLC、LC/MS/MS及1H-NMR等光譜分析，檸檬馬鞭草水萃取物製備出的純化物質，分別分離出具抗氧化及抑制醣解酵素作用之isoverbascoside (1)、verbascoside (2)、eukovoside (3) 、eukovoside isomer (4) 、ferulic acid (5)及caffeic acid(6)；而鐵馬鞭草同樣也分離出上述 1~6。 Diabetes are related to the insufficient insulin secretion or missing role related to the formation of the high blood sugar to the body produces too much and cause oxidation active oxygen pressure rise and related complications to occur, the oxidation material for active oxygen-caused disease-control plays an important role. The currency of the enzyme role not only in the human body digestion, such as diabetes and metabolic diseases many monetary-enzyme in the α-amylase and α-glucosidase enzyme is the most important Charter. Verbena officinalis L., also known as iron horse whip, has been proved to have antioxidant, anti-inflammatory, neuroprotective, analgesic, antifungal and other physiological functions, the laboratory has been from Verbena n-butanol extract as isoverbascoside (1)、verbascoside (2)、 eukovoside (3)、eukovoside isomer (4) and β-hydroxyverbascoside (5), But Verbena officinalis L. is not a kind of edible plant, which is not conducive to the development of health foods. The main purpose of this study is to study the effects of Verbena officinalis L. and Edible homologous Aloysia triphylla , Analysis and comparison of its antioxidant and glycolytic enzyme inhibitory active ingredients. The two species of Verbena were extracted by hot water (2 kg of hay / 100 L of water) and extracted by ultrasonic wave for 30 min, respectively. The crude extract was subjected to XAD-7 and ODS column chromatography, and then analyzed and prepared by HPLC. Finally, the antioxidant and glycoenzymes inhibitory active components were analyzed and compared by LC / MS / MS and 1H-NMR spectra. The results showed that the antioxidative activity of total extract, total flavonoids, total antioxidant, DPPH and anti-glycolysis α-glucosidase inhibitory activity of Aloysia triphylla extract was significantly higher than that of Verbena officinalis, α-amylase inhibitory activity No significant difference. Under the wavelength of 280nm, XAD-7 column chromatography region, Verbena officinalis distinguish I ~ V Division, Aloysia triphylla distinguish I ~ VI Division, and in Verbena officinalis III Division and Aloysia triphylla IV Division, its antioxidant activity and anti-glycolytic enzymes were significantly higher than other divisions; Aloysia triphylla IV of its antioxidant activity of total phenols, total flavonoids, total antioxidant capacity, DPPH Inhibition rate and α-glucosidase inhibitory activity were significantly higher than Verbena officinalis Division III, α-amylase inhibitory activity was not significantly different. The above distinction and the use of ODS column chromatography for further separation, the current known activity fell on the distinction between the first and II Division; and continue by HPLC, LC / MS / MS and 1H-NMR spectral analysis, The main purified substances of Aloysia triphylla with antioxidant and inhibit the role of enzymes saccharifying enzyme were identified as isoverbascoside (1)verbascoside (2)、eukovoside (3)、eukovoside isomer (4) and ferulic acid (5), caffeic acid (6) ; and Verbena also separated the above 1 ~ 6.
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