在心血管疾病中移植具有功能性的動脈非常迫切。在臨床上， 包括冠狀動脈與周邊血管，都需要小管徑的動脈移植。自體移植與異體移植血管的生物穩定性或是相容性在使用上須符合要求，但是這樣的來源是十分有限。而在製造人工血管的過程中，內皮細胞附著在胞外基質上是必要的先決條件。在我們的研究中，將以羊膜加工以獲得可支持內皮細胞的生長平台，並以黏著指數、反轉錄聚合連鎖反應、西方點墨法、免疫共同成沉澱與共軛焦顯微鏡去研究羊膜對於內皮細胞的影響。整合素為αβ相異二聚體之細胞底層表面受體，負責調控細胞的附著與抵抗血液的衝擊力。本實驗之目的將針對內皮細胞培養在羊膜上探討Integrin的表現與細胞黏著的影響。結果內皮細胞生長在羊膜上擁有正常內皮細胞的特色及外觀。在分子實驗的結果中，內皮細胞在羊膜上培養後，其整合素β1與活化態整合素β1的表現相對於沒生長在羊膜的內皮細胞，會有較高的蛋白質表現。細胞附著能力指數的結果，顯示黏著指數與整合素β1的表現是有正相關。 The requirements of functional artery replacement in cardiovascular diseases are urgent. Clinically, the small-diameter arteries are the standards for vascular grafts, including coronary artery bypass and peripheral vascular vessel replacement. The biostability of autografts and heterografts is satisfactory in clinical use, but the resource of the grafts is limited. Tissue-engineered blood vessels provide a promising way to generate suitable graft. The extracellular matrix is required for the adhesion of endothelial cells (ECs). Using amniotic membrane (AM), we manufactured a cell-free extracellular matrix scaffolds to obtain supportable material for endothelial cell growth. We used adhesion index, RT-PCR, western blot, immunoprecipitation, and confocal laser scanning microscopy to study the effects of AM on the endothelial cells. Integrins known as αβ-heterodimeric cell-surface basal layer receptors regulate cell adhesion and are essential for mechanotransduction of hemodynamic forces. Therefore, we investigated the effects of amniotic membrane (AM) on the expression of Integrin and EC adhesion. ECs cultured on AM exhibited normal features of the typical morphology. The molecular study of ECs cultured on AM showed a higher level of expressions in both forms of integrin β1 (integrin β1 and the active integrin β1) as compared to the ECs grown on dish without AM. We also followed the functional adhesion assay to analyze cells adhesion index. These results showed that the adhesion index and integrin β1 expression was directly related.
