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http://140.128.103.80:8080/handle/310901/3107
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| Title: | 液態培養條件對樟芝(Antrodiacinnamomea)菌絲體及多醣體之影響 |
| Other Titles: | Effect of submerged conditions on the mycelia and polysaccharides of Antrodia cinnamomea |
| Authors: | 王馨晨
Wang, Hsin-Chen |
| Contributors: | 顏文義
Yen, Wen-Yi
東海大學食品科學系 |
| Keywords: | 樟芝;多醣體;玉米澱粉
Antrodia cinnamomea;polysaccharide;β-1,3-glucan |
| Date: | 2007 |
| Issue Date: | 2011-03-30T06:14:15Z (UTC)
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| Abstract: | 樟芝(Antrodia cinnamomea)為台灣一種特有的藥用真菌，具有提昇人體免疫力及保肝的多種功能。野生牛樟生長緩慢且不易取得，近年來使用固態或液態培養，期能以較穩定的品質及較合理的價格供應保健食品市場的需求。本實驗採用液態培養，探討不同培養條件(不同碳源及氮源、不同碳源濃度及培養基不同初始pH值的調整) 以搖瓶培養及不同通氣量及攪拌速度在發酵槽培養，對樟芝菌絲體(mycelia)及多醣體(polysaccharides)的生產及多醣品質之影響。試驗結果顯示在搖瓶培養方面，以葡萄糖及玉米澱粉為碳源和以peptone及corn steep powder為氮源，採用玉米澱粉(3%)和peptone(0.5%)時，可得到最多菌絲乾重、胞外(EPS)及胞內多醣(IPS)分別為1.20 mg/ml 、17.48 mg/ml和0.22 mg/ml，及最佳多醣品質指標包含EPS及IPS之β-1,3-glucan含量分別為8.80 μg/ml LE、1.12 μg/ml LE及分子量3.76×105 Da；在不同碳源濃度試驗(1%、2%及3%)中，則以玉米澱粉2 %時，可得到最多菌絲乾重、胞外(EPS)及胞內多醣(IPS)分別為0.80 mg/ml、9.21 mg/ml及0.13 mg/ml，最佳多醣品質包含EPS及IPS之β-1,3-glucan含量分別為11.62 μg/ml LE及2.68 μg/ml LE；測試不同初始pH值時，以初始pH值未調整(約5.3)可得到最大菌絲乾重、EPS及IPS分別為0.85 mg/ml、14.02 mg/ml及0.07 mg/ml，最佳多醣品質包含EPS及IPS之β-1,3-glucan含量分別為11.06 μg/ml LE及2.54 μg/ml LE。在5公升的攪拌式發酵槽，進行不同的攪拌及通氣培養條件，以全程維持在100 rpm,1.0 vvm，的生產效果最佳，可得到EPS及IPS產量分別為22.06 mg/ml及 0.28 mg/ml，多醣品質指標包含EPS及IPS之β-1,3-glucan含量分別為21.81 μg/ml LE、2.92 μg/ml LE及分子量1.48×104 Da。
Antrodia cinnamomea is an important Chinese medicinal fungus , and is an unique species only grown in Taiwan. It has many functional properties in human body, especially for promoting immune system and protecting liver. As is growing slowly in the wild, supply of A. cinnamomea with stable product quality at reasonable price is in high demand. Research on the methods of artificial cultivation for this fungus is necessary. Our research is interest in attempting to utilize cheaper fermentation substrates for production of this organism, with emphasis on the productivity and quality of the microbial polysaccharides. This study was carried out by submerged fermentation of A. cinnamomea. Culture conditions which including different carbon and nitrogen sources, and different initial pH of fermentation medium in shake flasks. Effects of aeration and stirring in the fermentor were also investigated. The shake flask studies showed that using of corn starch and peptone combination as carbon and nitrogen sources had the best results in production of biomass, the contents of exo-polysaccharide (EPS) and intra-polysaccharide (IPS), with a concentration of 1.20 mg/ml, 17.48 mg/ml, 0.22 mg/ml, respectively in the fermentation broth. The content of β-1,3-glucan in EPS and IPS were 8.80 μg/ml LE, 1.12 μg/ml LE respectively, and had a molecular weight of 3.76×105 Dalton for EPS. Maximum results were obtained from the medium containing substrate concentration of 2% corn starch and unadjusted initial pH, where the biomass, EPS and IPS contents were 0.85 mg/ml, 14.02 mg/ml, 0.07 mg/ml, respectively, as compared to 1% and 3% starch and initial pH at 3, 4, 5 and 6. And β-1,3-glucan of EPS and IPS are 11.06 μg/ml LE and 2.54 μg/ml LE, respectively.The results from a 5 liter stirred tank fermentor ran with three different culture conditions showed that it was most suitable at 100 rpm, aeration rate 1.0 vvm throughout the fermentation period, with regard to production and quality of the polysaccharides. EPS and IPS were 22.06 mg/ml, 0.28 mg/ml, respectively. The β-1,3-glucan of EPS and IPS were 21.81 μg/ml LE and 2.92 μg/ml LE. The exo-polysaccharides had molecular weight of 1.48×104 Dalton. |
| Appears in Collections: | [食品科學系所] 碩士論文
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| 095THU00253006-001.pdf | | 126Kb | Adobe PDF | 433 | View/Open | | 095THU00253006-002.pdf | | 68Kb | Adobe PDF | 453 | View/Open | | 095THU00253006-003.pdf | | 2374Kb | Adobe PDF | 4186 | View/Open |
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