本研究以中藥材蔓荊子（seeds of Vitex trifloia L.）為實驗材料，主要探討抗氧化性與其抗氧化成分。實驗可分為兩部分，第一部分為中藥材之篩選，篩選材料為蔓荊子（seeds of Vitex trifloia L.）與蛇床子（seeds of Cnidium monnieri （L.）cuss），此兩種材料分別利用三種不同溶劑做萃取，結果顯示出蔓荊子甲醇萃取物的抗氧化力較佳於其他溶劑萃取與蛇床子各種溶劑萃取，故選取蔓荊子甲醇萃取物進行第二部分的抗氧化成分分析與結構鑑定。第二部分實驗分別利用XAD-7液相管柱層析與高效能液相層析儀(High Performance Liquid Chromatography ,HPLC)進行分離及純化，純化後之物質利用可見光-紫外線吸光光譜儀（UV-Visible spectrometer）、液相層析質譜儀（Liquid Chromatography Mass Spectrometer , LC-MS）及核磁共振儀（NMR）鑑定化學結構。結果顯示出蔓荊子甲醇萃取物以去離子水：甲醇（v/v）＝40：60及20：80（即Ⅵ、Ⅴ區分）之沖提條件下可獲得較好之抗氧化性，在Ⅵ、Ⅴ區分之抗氧化物質主要為luteolin 6-C-（6’’-O-trans-caffeoylglucoside）（VT-1）、luteolin（VT-2）、luteolin 7-O-（6’’-p-benzoylglucoside）（VT-3）、luteolin 7-O-β-sophoroside（VT-4）等四種luteolin及其衍生物。 The purposes of this study were investigating the antioxidative activity and antioxidative components of seeds from Vitex trifloia L. There are two parts in this experiment. The first, two kinds of Chinese herbs, seeds of Vitex trifloia L. and seeds of Cnidium monnieri （L.）cuss. were extracted with three different kinds of solvent . The antioxidative activity between seeds of Vitex trifloia L. and seeds of Cnidium monnieri （L.）cuss. was investigated and compared. The methanol extract seeds of Vitex trifloia L. which showed strong activity were isolated and purified by Amberlite XAD-7 and high performance liquid chromatography repeatedly . The structures of the isolated components were identified by UV-Visible spectrometer（UV）、 liquid chromatography mass spectrometer (LC-MS）and NMR. The main four components from methanol extraction of seeds from Vitex trifloia L. were identified as luteolin 6-C-（6’’-O-trans-caffeoylglucoside）（VT-1）、luteolin（VT-2）、luteolin 7-O-（6’’-p-benzoylglucoside）（VT-3）and luteolin 7-O-β-sophoroside（VT-4）etc.
