樟芝(Antrodia cinnamomea)為台灣特有的藥用菇類，已知主要的有效成分為多醣體、三萜類、固醇類及antroquinonol，其生理活性成分及功能仍是目前研究重點。本研究主要探討利用添加不同誘導子或不同濃度誘導子對樟芝菌絲體活性成分生成之影響，在三角瓶液態培養方面，比較不同誘導子再添加對活性成分含量之影響。在固態發酵方面，測試不同培養基比例與誘導子添加做饋料批式培養。在誘導子添加的實驗中，添加幾丁寡糖濃度為400 mg/L時，三萜類含量達到22.915 mg/g D.W.，為控制組的1.5倍，添加木質素時，無明顯誘導效果。在固態發酵培養實驗中，不同固態培養基試驗中60%豆渣+40%小米為培養基時有最好的培養效果。在誘導子部分分別添加紅蘿蔔、橘子、檸檬，三萜類含量方面，紅蘿蔔有最好誘導效果於第8周時達到34.14 mg/g D.W.，為控制組的2.26倍，antroquinonol方面，橘子皮有最好誘導效果於第8周時達到0.0585 mg/g D.W.，為控制組的2.71倍。 Antrodia cinnamomea has become a well-known medicinal mushroom in Taiwan. Triterpenoids and antroquinonol are considered to be the most biologically active components found in A. cinnamomea. The aim of this study was to investigate the effect of adding various elicitors on the formation of triterpenoids and antroquinonol. The results showed that chitosan oligosaccharide and carrot seemed to be promising elicitors for enhancing the production of triterpenoids. An addition of chitosan oligosaccharide of 400 mg/l can raise the crude triterpenoid content to the level of 22.92mg/g D.W., which is 1.5 times more than control group in submerged cultures. Moreover, adding carrot of 0.15 ml/g D.W. could effectively enhance the production of crude triterpenoid to the concentration of 34.14mg/g D.W., and was 2.26 times higher than the control in solid-state cultures. In contrast, tangerine peel was demonstrated to be effective in improving the formation of antroquinonol.
