| Abstract: | 本試驗探討以乳酸菌為添加劑對尼羅草青貯料發酵特性的影響。試驗使用的乳酸菌為Lactobacillus plantarum CCRC 12944菌液 ( LP ) ， Lactobacillus rhamnosus THSH-1菌液 ( LR )， 以及L. plantarum 與 L. rhamnosus兩種乳酸菌混合的混合菌液 ( LPR ) ，其濃度皆為106 CFU/g （新鮮草料重），及未添加菌液者為對照組（C）。於青貯後2到4天，LPR組之pH值下降速率最快且最低（P < 0.05），分別為3.21與3.46。青貯60天時，各處理之品質，pH值方面，以LP組最低（P < 0.05）；乾物質以LP組最高（29.5%），LPR組最低（25.2%）（P < 0.05）；氨態氮以C組最低（73.6 g/kg DM），LR組次之（74 g/kg DM）（P < 0.05）；水溶性碳水化合物、中洗纖維和酸洗纖維處理組間皆無顯著差異；酵母菌菌數在C組和LPR組皆小於2 log CFU/g，LP組和LR組分別為3.45 log CFU/g 和3.56 log CFU/g， 且四個處理組的黴菌菌數皆低於2 log CFU/g；有機酸方面，醋酸含量則是LR組最低，為16.23 g/kg DM ( P < 0.05 )，而丁酸含量為LP組最低，為0.95 g/kg DM ( P < 0.05 )；乳酸含量以LP組最高( P < 0.05 )，達28.73 g/kg DM。Flieg氏青貯料脂肪酸組以LP組分數最高，為70分，品質為四組中最佳者。
This study was conducted to determine the fermentation characterics of nilegrass silage inoculated with lactic acid bacteria. The inoculants contained L. plantarum CCRC 12944 (LP) , L. rhamnosus THSH-1 (LR) , and a mixture of L. plantarum and L. rhamnosus (LPR) (106 CFU/g FW) which were added to nilegrass, respectively, while the treatment without adding any inoculant was used as control (C) . After ensiling for 2 and 4 days, the pH values of LPR decreased faster than the other treatments ( P < 0.05 ), and these were 3.21 and 3.46. As to the quality of all silages after 60 days ensiling, LP had the lowest pH value ( P < 0.05 ) ; LP had the highest dry matter content ( 29.5% ), followed by LPR ( 25.2% ) ( P<0.05 )；ammonium nitrogen contents of C was 73.6 g/kg DM, which was the highest one, followed by that of LR ( 74 g/kg DM ) ( P<0.05 ) ; no significant effects were observed among the treatments for water soluble carbohydrates, neutral detergent fiber, or acid detergent fiber; after 60 days, the yeast numbers of C and LPR were below 2 log CFU/g, and the yeast numbers of LP and LPR were 3.54 log CFU/g and 3.56 log CFU/g respectively, the mold numbers of four treatments were below 2 log CFU/g；for the fatty acids, the acetic acid content of LR was 16.23 g/kg DM ( P < 0.05 ), and the butyric acid content of LP was 0.95 g/kg DM ( P < 0.05 ) . These were both the lowest treatments; the lactic acid content of LP was 28.73 g/kg DM ( P < 0.05 ), it was the highest one. Flieg’s score of LP was 70, and higher than those of the other treatments. It was concluded that using LP could make the best quality of silage. |
