鵝蛋黃蛋白質水解物抗氧化性之探討

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Title:	鵝蛋黃蛋白質水解物抗氧化性之探討
Other Titles:	Studies on Antioxidative Activities of Hydrolysates from Goose Egg Yolk Protein
Authors:	吳麗娟 Wu, Li-Jiuan
Contributors:	江文德;郭俊欽 Chiang, Wen-Dee;Kuo, Chun-Chin 東海大學食品科學系
Keywords:	鵝蛋黃蛋白質(GEYP)、酵素、抗氧化力、超濾膜過濾、胺基酸組成。 Goose egg yolk protein, Enzyme, Antioxidative activities, Ultrafiltration membrane, Amino acid composition.
Date:	2010
Issue Date:	2010-12-31T08:34:42Z (UTC)
Abstract:	本研究以7.5 % ( w/w of protein ) 之Alcalase 、Flavourzyme 及二種混合 (1:1) 之酵素 (Mix) 分別對8 % 鵝蛋黃蛋白質 (goose egg yolk protein; GEYP) 進行水解，並依水解時間製備0、0.5、4、8、12h水解物以探討水解時間 (hydrolysis time; HT) 與酵素種類對GEYP水解物之水解率 (degree of hydrolysis; DH)、產率及抗氧化特性之影響。結果顯示，三組酵素水解物之DH和產率皆隨HT增加而上升，其中以混合酵素水解GEYP12h所得之水解物 (MH12h) 其DH最高可達56.7 %，其次為Flavourzyme 的36.7 %。就抗氧化性而言，MH12h具最佳的α, α-diphenyl-β-picrylhydrazyl (DPPH)自由基清除能力、氫氧自由基清除能力、還原力及亞鐵離子螯合力。將MH12h依序經30、10、1及0.15 kDa分子量限值濾膜進行區分，分別可得30、10、1與0.15 kDa濃縮液與0.15 kDa濾液，30 kDa 濃縮液具最佳的亞鐵離子螯合力與DPPH自由基清除能力，此區分物之Tyr與Ser含量顯著增加，這可能與其抗氧化力之提升有關。1 kDa濃縮液具有最佳的還原力與清除氫氧自由基的能力，可能與胺基酸Pro含量增加有關。上述兩區分物之分子量主要小於1,000 Da，推測此分子量之胜?提供較佳的抗氧化力。由此可知MH12h有良好的抗氧化力，未來具開發為天然抗氧化物質應用於食品系統中之潛力。 The purpose of this study was to investigate the effects of hydrolysis time (HT) (0, 0.5, 4, 8, 12 h) and enzyme types (Alcalase, Flavourzyme, and Mixed enzyme Alcalase + Flavourzyme) on degree of hydrolysis (DH), yield, and antioxidant activities during hydrolysis of 8 % goose egg yolk protein (GEYP). Each enzymes concentration was 7.5% (w/w of protein). Both DH and yield of the hydrolysates increased with increase of HT. The hydrolysate obtained from hydrolysis of GEYP with mixed enzyme for 12h (MH12h) had the highest DH of 56.7 % than Flavourzyme’s 36.7 %. For antioxidative activities of the hydrolysates, MH12h had the strongest α, α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging activity, hydroxyl radical scavenging activity, reducing power, and ferrous ion chelating activity. MH12h were further factionated by ultrafiltration membranes with 30, 10, 1, and 0.15 kDa molecular weight cut-off (MWCO) sequentially to obtained 30, 10, 1, 0.15 kDa concentrate and 0.15 kDa permeate, respectively. The result showed 30 kDa concentrate had higher ferrous ion chelating activity and DPPH radical scavenging activity than other fractions. For the amino acid composition, 30 kDa concentrate had higher Tyr and Ser. 1 kDa concentrate had higher reducing power and hydroxyl radical scavenging activity than others. It might be due to its higher Pro content. The molecular weight profile of 30 kDa and 1 kDa concentrate had mainly 1,000 Da. Therefore, MH12h had stronger antioxidative activity , and may serve as potential nature antioxidative materials for food system in the future.
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