不同菇類菌絲體萃取物ACE抑制活性之探討

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Title:	不同菇類菌絲體萃取物ACE抑制活性之探討
Other Titles:	Effect of mycelial extracts from different mushroom on ACE inhibitor activity
Authors:	葉怡妏 Ye, Yi-Wen
Contributors:	楊芳鏘 Yang, Fan-Chiang 東海大學化學工程與材料工程學系
Keywords:	朱紅栓菌;ACE抑制劑;菇類菌絲體;抗高血壓 P. cinnabrinus;ACE inhibitor;mycelium of mushrooms;antihypertension
Date:	2010
Issue Date:	2011-01-03T05:34:22Z (UTC)
Abstract:	本研究探討四種食藥用菇類菌絲體（朱紅栓菌、靈芝、樟芝與雞腿蘑）萃取物的ACE抑制活性功能，期待以此開發出新的抗高血壓保健食品。本研究方法先是分別以深層培養與固態培養方式得到菌絲體，並製成不同溶劑之萃取物與酵素水解物，另外再探討朱紅栓菌與雞腿蘑之自體溶解反應，測定ACE抑制活性。研究結果顯示，當以深層發酵方式培養菌絲體時，四者皆是以冷水萃取物有較高的ACE抑制活性，分別為69.4%、57.2%、26.8%與15.2%，其IC50則為2.45、4.13、9.28與13.5 mg/ml，而乙醇萃取物是無抑制活性。在固態培養方面，選用了薏仁、燕麥與蕎麥作為培養基質，朱紅栓菌、靈芝與樟芝以薏仁為培養基質時有最高之抑制活性，雞腿蘑則以燕麥培養時有最高之抑制活性。探討朱紅栓菌之自體溶解反應對抑制活性的影響，其最佳作用環境為溫度為50℃、pH 4。另外，選用papain、bromelain與alcalase水解朱紅栓菌菌絲體探討其ACE抑制活性，結果顯示alcalase作用效果最佳，其抑制活性可達82.3%。由以上結果得知，朱紅栓菌比其他三者有較高之ACE抑制活性，可作為日後開發保健食品之另一項新選擇。 The purpose of this study was to investigate the effect of the extracts with different solvents and autolysis treatment of mycelia of medicinal mushroom (P. cinnabarinus, G. lucidum, A. camphorate and C. cinereus）on ACE inhibitory activity and expected to develop a new healthy food for regulating blood pressure. The mycelia were produced by submerged cultures or solid-state fermentation using different substrate. Based on the results, when cold water was used for extraction, higher ACE inhibitor activities were obtained, which were 69.4%、57.2%、26.8% and 15.2% for P. cinnabarinus, G. lucidum, A. camphorate and C. cinereus, respectively. Moreover, the IC5 values were determined as 2.45、4.13、9.28 and 13.5 mg/ml. The maximum ACE inhibitor activity was obtained when the mycelium was extracted using water. The ethanol extract showed no ACE inhibitor activity. Pearl barley, oats and buckwheat were used as substrate for solid-state fermentation. When pearl barley was used as a substrate, the mycelium extracts of P. cinnabarinus, A. camphorate and G. lucidum had the higher inhibitor activity, however oats was a more suitable substrate for the culture of C. cinereus.. Concerning the autolysis of P. cinnabarinus , the optimum condition was determined to be a temperature equal to 50℃ and pH 4. When commercial proteases (papain, bromelain and alcalas) were employed for the mycelia hydrolysis of P. cinnabrinusn, alcalase was demonstrated to achieve the highest ACE inhibitor activity of 82.3%.
Appears in Collections:	[化學工程與材料工程學系所] 碩博士論文

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