以分散式液液微萃取技術結合基質輔助雷射脫附游離飛行時間質譜儀分析毛花苷化合物

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題名:	以分散式液液微萃取技術結合基質輔助雷射脫附游離飛行時間質譜儀分析毛花苷化合物
其他題名:	Using Dispersive Liquid-Liquid Microextraction Method for the Determination of Lanatoside C by Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.
作者:	籃品渝 Lan, Pin-Yu
貢獻者:	劉建忠 Liou, Chien-Chung 東海大學化學系
關鍵詞:	分散式液液微萃取法、雷射脫附游離飛行時間質譜儀、強心配醣體、萃取劑、分散劑 dispersive liquid-liquid microextraction method, MALDI-TOF MS, Cardiac Glycoside, extraction solvent, dispersive solvent
日期:	2010
上傳時間:	2011-01-03T05:39:35Z (UTC)
摘要:	本論文提出一項新的分析技術，以分散式液液微萃取法結合雷射脫附游離飛行質譜儀，對強心配醣體藥物毛花苷進行鑑定分析。　　利用分散式液液微萃取技術，可以同時達到萃取與濃縮分析物的效果，將萃取劑(碘甲烷)與分散劑(四氫呋喃)，快速注入樣品溶液中，形成混濁狀的溶液，此時分析物通過分散劑被萃取至萃取劑中，經由離心將沉積相分離，取出離心管底部的沉積液並且與基質溶液 α-CHCA混合，以基質輔助雷射脫附游離飛行質譜法進行分析物的偵測。實驗中探討萃取劑的種類與體積、分散劑的種類與體積、pH值、萃取時間、離心時間等因素對於實驗結果的影響，在最佳化的實驗條件下，所得到的分析物檢量線，濃度線性範圍為 0.01 μM 至1 μM，相關係數（R2）為0.9911，偵測極限可達到1.06 nM。　　將此分析方法應用於人體尿液樣品的偵測，可成功地檢測出毛花苷化合物的成份，偵測極限為13.1 nM。另外，此分析方法也可進一步應用於檢驗尿液樣品中，含有多種強心配醣體藥物(毛地黃毒苷、毛地黃素和毛花苷)，達到同步偵測之目的。 Lanatoside C, a kind of cardiac glycoside was determined by a novel dispersive liquid-liquid microextraction method coupled with MALDI-TOF MS. The appropriate mixture of extraction solvent(CH3I) and dispersive solvent(THF)was rapidly injected into a sample solution. It formed a cloudy solution and Lanatoside C was extracted into the extraction solvent by the dispersive solvent. After centrifugation, the sediment solvent was mixed with α-CHCA before determined by the MALDI-TOF MS. Several important experimental condition, such as the kind and the volume of extraction solvents, dispersive solvent, extraction time and centrifugation time were investigated. Under the optimal conditions, our experiment results showed a linear calibration curve in the concentration ranged from 0.01 μM to 1 μM with a correlative coefficient(R2)0.9911 and the limit of detection for a standard solution of Lanatoside C was 1.06 nM. This method had been successfully applied for the analysis of Lanatoside C in urine. In addition, a mixture of Digitoxin, Digoxin and Lanatoside C solution could be analysed simultaneously.
顯示於類別:	[化學系所] 碩博士論文

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