谷關溫泉中三嗜熱厭氧木質素降解細菌之分離定性及鑑定

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Title:	谷關溫泉中三嗜熱厭氧木質素降解細菌之分離定性及鑑定
Other Titles:	Isolation, characterization and identification of three lignin-degrading thermophilic anaerobic bacteria from Guguan hot spring.
Authors:	林立僑 Lin, Li-Chiao
Contributors:	黃啟裕 Huang, Chi-Yu 東海大學環境科學與工程學系
Keywords:	嗜熱細菌;厭氧細菌;木質素生物降解 Thermophile;anaerobe;lignin-degrading
Date:	2003
Issue Date:	2011-05-19T06:33:16Z (UTC)
Abstract:	本研究由台中縣谷關溫泉中採得底泥及溫泉水中分離純化出具木質素降解潛力之厭氧嗜熱菌株，經初步對木質素之色度降解能力以及16S rRNA gene sequence定序比對結果，篩選出三菌株K1L1、K2L1及K3L1更進一步探討此三菌株之生化特性。三研究菌株K1L1、K2L1及K3L1均為革蘭氏陰性桿菌，並且均為絕對厭氧菌株。其最適生長條件分別為45℃、pH 7.4；60℃、pH 6.4；55℃、pH 7.4。菌株K1L1、K2L1具移動性；而K3L1不具移動性，但會生成孢子。在木質素降解方面，三菌株對鹼水解之木質素分別可達62.3%、57.1、61.0% 之脫色效率。並且可改變可溶性木質素之分子量分布以及利用木質素代謝產生乙酸。由16S rDNA分析結果顯示，已知菌株Thermophilic bacterium JA2與K1L1有最高之序列相似性，但僅達89%相似度，與質基利用、抗生素感受性、醱酵產酸測試、酵素測試等生化特性相對照，K1L1極可能為新種或新屬之菌株。而K2L1及K3L1比對之結果均屬於Caloramator sp.，根據此二菌株之生化特性，亦可能為此屬中之新種菌株。 Several thermophilic anaerobic lignin-degrading bacteria were isolated from Guguan hot spring. According to decoloration of lignin solution and 16S rRNA gene sequencing, strains K1L1, K2L1 and K3L1 were chosen for further study of their biochemical characteristics. Strains K1L1, K2L1 and K3L1 were gram negative, strict anaerobe. The optimal growth conditions are 45℃, pH 7.4; 60℃, pH 6.4; 55℃, pH 7.4 respectively. Strains K1L1 and K2L1 were motile while K3L1 was non-motile. Strain K3L1 was a spore-forming bacterium. As for lignin degradation, the decolorations efficiency of alkali-hydrolyzed lignin for the three strains were 62.3%, 57.1% and 61.0%. On the other hand all these three strains could change the molecular weight distribution of soluble lignin and metabolized lignin to produce acetic acid. According to 16S rDNA analysis, strain Thermophilic bacterium JA2 was the most similar one to strain K1L1, but the similarity was only 89%. Compared with other biochemical characteristics such as the utilization of substrates, antibiotic resistance, fermentation metabolite test and enzyme test, strain K1L1 was a novel specie of thermophilic anaerobe. Strains K2L1 and K3L1 belonged to Caloramator genus. According to some characteristics, both strains may be the species belonging to Genus Caloramator.
Appears in Collections:	[環境科學與工程學系所] 碩博士論文

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