β3-腎上腺素激導性接受器(β3-adrenergic receptor; 齒類動物解脂作 用之主要接受器。而內泌素解脂■(Hormone- sensitive 關鍵酵素。 本論文之目的為探討絕食和isoproterenol注射對β3-AR和HSLA以及再餵 食時的反應。以反轉錄聚合■連鎖反應(Reversepolymerase chain reaction;測脂肪、肌肉和肝臟組織中β3-AR與HSL的mRNA含量。試驗一， 絕食與再餵MHSL基因表現的影響。試驗二，探討β-adrenergic鼠β3-AR 和HSL基因表現之影響。試驗三，分離豬的β3-AR和HSL基因，以允齔揚劂 P再餵飼的反應。結果顯示，由於大鼠不同組織中β3-AR和HSL對絕瓣ㄓ@ 致，因此營養供給所引起β3-AR和HSL梒握妖S異性。而脂肪和肝臟中β3- AR和HSL基因表現對isoproterenol的反酮O因調節機制不同所致。絕食與 再餵食對大鼠與豬不同組織中HSL基因的蔡均C Catecholamines stimulate lipolysis mainly through β3- adrenergicRs) in adipose tissue of rodents. Hormone-sensitive lipase (HSL)iting enzyme of triglyceride hydrolysis. To investigate theitional tranisition and isoproternol injection on β3-AR and HSL, mRNA levels of β3-AR and HSL were examined in adipose tissue,tal muscle by the method of reverse transcription-polymerase(RT-PCR). In experiment 1, the effects of fasting and refeeding on β3-ARpression were examined in male Wistar rats. In experiment 2, theto investigate the effect of isoproterenol injection on β3-ARpression in rats. In experiment 3, partial porcine β3-AR and HSLed to be isolated, and their gene expression in response toeeding was examined in Landrace pigs. These results showed thatof rat β3-AR and HSL gene expression by nutritional alteration and β- adrenergictissue-specific. No β3-AR gene expression was detected in pigThe sequences of HSL cDNA was conserved between rats and pigs,sion in pig tissue in response to fasting and refeeding wasthat in rats.
