以動物為模式測試豬胸膜肺炎放線桿菌疫苗之保護效果

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题名:	以動物為模式測試豬胸膜肺炎放線桿菌疫苗之保護效果
其它题名:	The Aminal Model in Evaluating the efficiency of Actinobacillus Pleuropneumoniae Vaccine
作者:	陸佩芝 Lu, Pei-Chi
贡献者:	劉琳琳, 翁仲男 Yun-Chu Wu 東海大學畜產與生物科技學系
关键词:	豬胸膜肺炎放線桿菌;疫苗 actinobacillus pleuropneumoniae;vaccine
日期:	1996
上传时间:	2011-05-24T07:59:18Z (UTC)
摘要:	本實驗目的在找出最適合胸膜肺炎放線桿菌 (App.) 生長條件，分析造成過敏反應的原因並測試 App. 疫苗對豬隻的保護效力。實驗使用四種培養基，含 1% NAD 的大豆胰蛋白培養基 (TS)、含 5% Yeast Extract (YE) 的 TS、含 1% NAD 的腦心萃取培養基 (BHI) 及含 5%YE 的 BHI 。在菌量上以含 1% NAD 的 BHIB 及含 1% NAD 的 TS 菌量最高。在溶血素產量上，以含 1% NAD 的 TS 力價最高 (256H.U.)，而含 5% YE 的 BHI 力價最低 (4H.U.)。四種培養基在酸鹼值的變化曲線相似，均於第 4 小時酸鹼值 (pH) 大幅由 7 下降至 6 左右。由實驗結果選擇含 1% NAD 的 TS 作為 App. 的最佳培養基。以菌液、無菌上清液、菌體、外毒素、內毒素與鋁膠分別腹腔接種小鼠以分析 App. 疫苗過敏因子。結果發現菌體、內毒素與外毒素均可引起過敏反應，但經過福馬林不活化處理後可去除菌體與外毒素的過敏反應，但內毒素的過敏反應則無法以福馬林不活化處理去除。以口服 10ml、口服 20ml、針劑與口服 20ml 及針劑注射等四種疫苗免疫方式免疫豬隻測定 App. 疫苗保護效果。結果在免疫組的血清、肺腸洗液並無抗體力價上升，僅在針劑組有部分的保護效果 (33%)。 The objectives of this study were to investigate the best growth condition for App and to evaluate the efficiency of App vaccine in pig. Among these four medium, TS 1%NAD, TS 5%Yeast extract (YE), BHI 1%NAD and BHI 5%YE, App had the highest bacterial count and hemolycin titer (256h.U.) when cultured in TS 1%NAD. The changes of pH curve were similar in four different medium. After 4 hours incubation, the pH value decreased from 7 to 6. To investigate the allergic component of App, bacterial culture, bacteria -free culture medium, bacterial pellet, exotoxin, endotoxin and alumina hydroxide (adjuvent) were injected into mice. The results showed that bacterial pellet, exotoxin and endotoxin induced anaphylaxis in mice. After treating these three allergic components, bacterial pellet and exotoxin failed to induced anaphylaxis whereas endotoxin remained the capacity of inducing anaphylaxis in mouse. To evaluate the efficiency of the App vaccine in the pig, four different methods of administration, oral 10ml, oral 20 ml, I. M. 20ml and oral and I.M. combination, were used to immunize pigs. After challenged with App, all of the immunized pigs died within 24 hours. The antibody titers did not increase in serum and intestinal mucus of immunized pigs. These results imply that this App vaccine fails to protect pigs from App infection.
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