本研究利用植物組織培養法探討不同品系紫田薯（Dioscorea alata L. var. purpurea M. Pouch）薯塊之癒合組織誘發及繼代培養條件、並對薯塊及癒合組織所含色素之安定性及品質進行探討。 不同品系紫田薯薯塊培植體誘發癒合組織之誘發率與誘發程度以添加30μM 2,4-D最佳，而kinetin 或 BA之添加對各品系癒合組織間之誘發率或誘發程度並無顯著差異。而蔗糖濃度方面，各品系紫田薯以添加1.01﹪蔗糖於MF-1培養基中癒合組織誘發率與誘發程度最高。添加GA3於MF-1培養基對癒合組織誘發、生長及繼代培養有抑制效果。而不同基礎培養基如Heller、 B-5、WPM及White等四種基礎培養基均無法誘發癒合組織，而MS培養基及其修飾之MF-1培養基可誘發癒合組織，繼代培養後，癒合組織產生褐變。 各品系紫田薯薯塊及癒合組織花青素方面，在不同pH值下之可見光吸收光譜類似，隨pH的提升，吸收峰由一個改變至兩個，顏色變化為：黃紅色 →淡紅色→紅紫色→紫色→藍紫色。薯塊花青素加熱 60 分鐘後，殘留率可達91﹪，癒合組織花青素殘留率達80﹪。而各品系薯塊與癒合組織花青素均具有良好的紫外光安定度，殘留率均有85﹪。 The initiation, growth and subculturing of calli from tuber explants of different lines of Dioscorea alata L. var. purpurea M. Pouch were studied. The stability and quality of the pigments in the tuber explants and the initiated calli were also investigated. The addition of 30μM 2,4-D induced more calli than other combinations from the tubers of different lines of Dioscorea alata L. var. purpurea M. Pouch. The addition of kinetin or BA did not significantly affect the percentage or the degree of callusing. The addition of 1.01﹪sucrose to the MF-1 media initiated the highest percentage or the degree of callusing. However, adding GA3 to the MF-1 media did inhibit the initiation and growth and subculturing of calli. Different basic media such as Heller, B-5, WPM, and White did not initiate calli. The MS or its modified one, the MF-1 did initiate calli, however, the calli became brown after subculturing. The absorption spectrums of anthocyanins from different lines of Dioscorea alata L. var. purpurea M. Pouch and at various pH were similar to one another. With the increase of pH, the number of absorption peaks increased from one to two. The change of color was from yellow-red to red to red-violet to violet and to blue-violet. After the tuber anthocyanins were heated under 95 ℃ for 60 minutes, its retention was 91%. For the anthocyanins from the calli the retention was 80% . The anthocyanins from tuber and calli showed good UV stability with the retention of 85% .
