目前消費者對於基因改造生物（Genetically Modified Organisms；GMO）之品質充滿疑慮，本研究目的在探討一般市售台農二號木瓜及附有木瓜輪點病毒的轉鞘蛋白基因台農二號木瓜之物理品質差異，另一目標是比較分析轉鞘蛋白基因台農二號木瓜果實中鞘蛋白基因之表現，以期消除消費者之疑慮。 在果皮及果肉色澤方面，非轉基因木瓜果皮亮度比轉基因木瓜果皮較高且有顯著差異。而非轉基因木瓜果皮之紅色度、黃色度及色彩飽和度比轉基因木瓜果皮高，但均無顯著差異。 在質地輪廓分析方面，非轉基因木瓜之破碎度、硬度、彈性、咀嚼性及膠性均比轉基因木瓜高。以刀刃狀探頭及擬齒狀探頭進行質地分析，結果顯示在硬度上，非轉基因木瓜及轉基因木瓜有明顯差異，在作功上則無差異。 以PCR可以檢測出經基因改造之木瓜，而ELISA及Western blot analysis則不能檢驗出來。在檢測PRSV方面，以RT-PCR能獲得最好的檢測結果。 在蛋白質濃度分析，發現轉基因木瓜與非轉基因木瓜並無顯著差異。在鞘蛋白分析方面，以ELISA分析、Western blotting分析及??圖譜比對後，發現轉基因木瓜及非轉基因木瓜並無顯著差異。 本實驗結果顯示，轉基因木瓜在物理品質上與非轉基因木瓜差異並不大，可視為兩者實質同等。因此，經此實驗可初步判定，轉基因木瓜與非轉基因木瓜應無食用安全上之疑慮。 Nowadays, the consumer is suspicious of the quality of Genetically Modified Oraganism. This research focuses on the difference of physical qualities between Tai-Nong No.2 papaya and transgenic Tai-Nong No.2 papaya conferred by the coat protein gene of Papaya Ringspot Virus. Another goal of this investigation was to study the expression of the coat protein gene of transgenic papaya in order to diminish the doubt of the consumer about transgenic papaya. In respect of peel and pulp, the lightness(L*) of the peel in non- transgenic papaya was higher than that of transgenic papaya, but the redness(a*)、yellowness(b*) and chroma of the peel was not significanly different. In respect of T.P.A., the fracturability、hardness、springiness、chewiness and gumminess of non-transgenic papaya was higher than transgenic papaya. By using blade set probe and Volodkevich bite jaws probe to analyse texture, the result showed that the hardness was significantly different between non-transgenic and transgenic papaya, but there was no difference in work. By means of PCR method, we could detect the CP transgene in transgenic papaya, but we couldn’t get the same result by ELISA and Western blotting analysis. In respect of PRSV, the RT-PCR showed the best detection. The protein concentration of transgenic papaya and non-transgenic papaya was not significanly different. In respct of coat protein, by using ELISA, Western blotting and peptide mapping analysis, we found that there was no difference between transgenic papaya and non-transgenic papaya. In conclusion, the physical quality in non-transgenic papaya and transgenic papaya is substanstially equivalent. Therefore, the transgenic papaya could be regarded as safe.
