B型肝炎病毒 e 蛋白的免疫調節作用

Tunghai University Institutional Repository > 理學院 > 生命科學系所 > 國科會研究報告 > Item 310901/8936

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Title:	B型肝炎病毒 e 蛋白的免疫調節作用
Other Titles:	Immunomodulatory Function of the e Protein of Hepatitis B Virus
Authors:	胡承波 Hu, Cheng-Po
Contributors:	行政院國家科學委員會東海大學生命科學系
Date:	2007
Issue Date:	2011-06-15T09:46:15Z (UTC)
Abstract:	B型肝炎病毒(HBV)造成慢性感染的原因不明，可能與HBV的e (HBe) 蛋白有關。本實驗室過去發現HBe可與人類血液單核細胞及小鼠巨噬細胞結合，並刺激產生細胞激素。巨噬細胞亦可將抗原呈現給T細胞，促使T細胞增生。本研究之目的在於探討HBe蛋白是否影響T細胞功能。結果顯示(1) HBe本身並不刺激細胞分裂，但在ConA的存在下，會增加小鼠脾臟細胞分裂，(2)在利用anti-CD3及anti-CD28抗體活化T細胞的實驗中，HBe會促進CD8+ T細胞分裂，但對CD4+ T細胞卻降低其分裂能力，(3)利用T細胞受體基因轉殖小鼠OT-1來探討T細胞增殖反應時，如果預先將HBe蛋白與抗原呈現細胞在試管中培養，則可使OT-1抗原專一性的CD8+ T細胞分裂增加。由本研究的結果看來，HBe蛋白可能對T細胞功能有某些程度的影響，此發現再次顯示HBV與宿主免疫反應之間具有相當複雜且微妙的相互作用。 Hepatitis B virus (HBV) infection is a global public health problem. Approximately one million people die from HBV-related liver cirrhosis and hepatocellular carcinoma every year. In order to persist in the host, HBV is believed to have immunomodulatory abilities to weaken the host immune response. However, the mechanism of chronicity of HBV infection is still unknown. The hepatitis B viral e (HBe) protein is a non-structural protein. It is not present in the virion and not required for infection or replication of HBV. Since the HBe protein is conserved in all members of hepadnaviruses, it is suggested to play a role in HBV-host interactions. Our laboratory has previously found that the HBe protein not only binds to human blood monocytes but also mouse macrophages from the spleen, the abdominal cavity and the liver. Furthermore, the HBe protein modulates cytokine release, chemotaxis and the production of peroxides from the cells of the 1st-line defense. In addition to innate immune functions, macrophages also present antigens to T lymphocytes, leading to T cell activation and proliferation. The purpose of this research is to study whether the HBe protein modulates T cell responses, either directly or indirectly through antigen-presenting cells (APC). The results show that (1) the HBe protein itself does not affect T cell proliferation, however, it enhances splenocyte division in the presence of a T-cell mitogen, concanavalin A, (2) in the anti-CD3 and anti-CD28 crosslinking experiments, the HBe slightly increases the proliferation of CD8+ T cells, but decreases that of CD4+ T cells, and (3) pre-incubation of the HBe protein with APCs enhances the proliferation of antigen-specific CD8+ T cells obtained from T cell receptor (TCR)-transgenic mice, OT-I. The findings of this study suggest that the HBe protein may affect APCs, CD4+ T cells and CD8+ T cells. These results further imply a complicated and subtle interaction between HBV and the host.
Relation:	研究編號:NSC95-2320-B029-001-MY3 研究期間:2007-08~ 2008-07
Appears in Collections:	[生命科學系所] 國科會研究報告

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