Prox1對Ff1b於班馬魚Adrenal/Interrenal發育過程中之輔助調控(III)

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Title:	Prox1對Ff1b於班馬魚Adrenal/Interrenal發育過程中之輔助調控(III)
Other Titles:	The Coregulation of Ff1b by Prox1 in the Adrenal/Interrenal Development of Zebrafish (III)
Authors:	劉薏雯 Liu, Yi-Wen
Contributors:	行政院國家科學委員會東海大學生物系
Keywords:	斑馬魚, 腎間腺, 內皮細胞 Zebrafish, Interrenal tissue, Endothelium
Date:	2005
Issue Date:	2011-06-17T06:30:04Z (UTC)
Abstract:	腎上腺皮質擁有複雜的血管系統, 這些血管對於組織的生長, 組織的維持, 以及類固醇類的釋放至循環系統佔有不可或缺的角色. 然而, 在早期腎上腺皮質器官發生的過程中, 血管系統和腎上腺組織如何進行交互作用, 是一未知而尚待研究的領域. 我們早先的研究中曾分離並鑑定一個斑馬魚的孤獨核接受器(orphan nuclear receptor) 基因ff1b (nr5a4), 研究 ff1b 基因的表達引導我們發現斑馬魚中相當於哺乳類腎上腺的器官---腎間腺 (Chai et al; 2003; Hsu etal, 2003). 雖然斑馬魚的腎間腺及哺乳類的腎上腺存在一些差異, 然而在早期器官的種種發生機制, 諸如細胞移行, 細胞特化, 細胞增殖及細胞分化等, 腎間腺及腎上腺擁有極強的相似性.斑馬魚腎間腺的早期發育, 在空間及時間上與原腎 (pronephros) 呈現高度相關, 並與內皮細胞緊密接合. 由於早期斑馬魚胚胎並不依靠血液循環來輸送氧氣, 我們可以在毋須考慮缺氧副作用的請況下, 利用斑馬魚來研究內皮細胞缺陷對於胚胎發生及器官發生所產生的效應.在本研究中, 我們使用了不同的研究策略包括血管突變種斑馬魚, 以及以antisense-morpholino核酸為本的專一性基因表達剔除技術, 探討內皮細胞在早期腎間腺器官發生過程中所扮演的角色. The adrenal cortex has a complex vasculature that is essential for growth, tissue maintenance, and access of secreted steroids to the circulation. However, the interaction between the vascular system and adrenal cortex during early organogenesis has remained largely unclear. The previous identification of orphan nuclear receptor ff1b (nr5a4) has led to the characterization of zebrafish interrenal organ, the functional counterpart of mammalian adrenal gland (Chai et al; 2003; Hsu et al, 2003). Despite the undeniable species-specific differences, the relevance of zebrafish interrenal to the mammalian adrenal organogenesis may reside in some of the developmental modules including migration, specification, proliferation and differentiation. The interrenal tissue develops in parallel with pronephros at the early stages, and displays a tight association with the endothelial cells. As early zebrafish embryo does not depend on vasculature to transport oxygen, it is an ideal model to explore the effects of endothelial dysfunction to embryogenesis and organogenesis, precluding the confounding factor of hypoxia. The role of endothelial signaling in the interrenal development was explored in this study through a combination of approaches including genetic mutant and morpholino-based knockdowns. The early interrenal primordia emerge as two clusters of cells that migrate centrally and converge at the midline, whereas the central convergence was abrogated in the avascular cloche (clo) mutant. Neither loss of blood circulation nor perturbations of vessel assembly could account for the interrenal convergence defect, implying a role of endothelial signaling prior to the formation of axial blood vessels. Moreover, as the absence of trunk endothelium in clo mutant was rescued by the forced expression of SCL, the interrenal fusion defect could be alleviated. We thus conclude that endothelial signaling is involved in the morphogenetic movement of early interrenal tissue.
Relation:	研究編號：NSC94-2320-B029-001 研究期間：2005-08 ~ 2006-07
Appears in Collections:	[Department of Life Sciences ] National Research Council Report

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