以硒化鎘量子點電化學發光生物感測器分析嘉磷塞與人體血清儲鐵蛋白的研究

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Title:	以硒化鎘量子點電化學發光生物感測器分析嘉磷塞與人體血清儲鐵蛋白的研究
Other Titles:	Studies on the analysis of glyphosate and human serum ferritin by CdSe quantum dots electrochemiluminescence biosensors
Authors:	郭家瑜 Kuo, Gu-Yu
Contributors:	黃承文 Whang, Cheng-Wen 東海大學化學系
Keywords:	生物感測器;儲鐵蛋白;嘉磷塞;硒化鎘量子點 ferritin;glyphosate;CdSe QDs;biosensor
Date:	2011
Issue Date:	2011-10-12T07:37:56Z (UTC)
Abstract:	無機半導體奈米晶體一般通稱為量子點（quantum dots, QDs）。與塊材不同，QDs的物理及化學性質取決於粒子的大小，具有特殊光學性質，是一種良好的發光材料。網版印刷電極相較於傳統固態電極具有低成本、製作簡單、不易鈍化及可拋棄等優點，因此在化學分析上更具實用性。本研究探討結合QDs電化學發光及網版印刷碳電極發展偵測嘉磷塞農藥（glyphosate；Gly）及人體血清儲鐵蛋白（ferritin）的生物感測器。先將碳奈米管與甲殼素混合溶液塗覆於碳電極表面，形成導電高分子膜，再將表面修飾thioglycolic acid （TGA）的CdSe/TGA QDs固定於薄膜內，最後以(3-aminopropyl) triethoxysilane（APS）及N-succinimidyl-4-(maleimidomethyl)- -cyclohexanecarboxylate （SMCC）當作交聯劑將分析物抗體連結至QDs表面。當樣品溶液中的分析物抗原與電極上抗體結合時，會造成QDs表面狀態改變，降低QDs的ECL強度，由ECL的衰減程度可定量樣品中的分析物抗原。本論文分為兩部分：第一部份以QDs結合生化免疫修飾網版印刷碳電極偵測Gly，檢量範圍為0.01?20.0 ng/mL，線性相關係數(R2) 0.9906。第二部分為更換網版印刷碳電極表面QDs修飾的抗體以偵測人體血清中的ferritin。Ferritin的檢量範圍為0.1?20.0 ng/mL，線性相關係數(R2) 0.9952，偵測極限0.07 ng/mL。探討血液中5種可能的干擾物，包括：人血清白蛋白、甲型胎兒蛋白、血紅蛋白、輸鐵蛋白、氯化鐵對本方法偵測之影響，結果顯示干擾影響甚微。比較本方法和 ELISA方法分析10個志願者血清中的ferritin含量，結果相當一致，顯示本研究發展的ferritin感測器具有高靈敏度及準確性，同時具有成本低廉及可大量製備等優點。 This studies has discussed to modify CdSe quantum dots (QDs) on screen-printed carbon electrodes, and conjugate to antibody detected glyphosate and human serum ferritin, developing a QDs electrochemiluminescence (ECL) biosensor. First a carbon Nanotube (CNT) and chitosan (CHIT) mixture coating on electrode surface, forming a CNT-CHIT thin film , then the surface modified thioglycolic acid (TGA) CdSe/TGA QDs is fixed in the thin film. use (3-Aminopropyl)triethoxysilane (APS) and N-succinimidyl-4-(maleimido- -methyl) cyclohexanecarboxylate (SMCC) to link antibody with the QDs on electrode surface. When antigen in sample solution reacted with antibody producing immunocomplex , will cause changed of the QDs surface state , result in ECL intensity decayed. This dissertation has two parts: The first part detects Gly by the QDs cajugated Gly antibody modified screen printing carbon electrode, examines the mensurable range is 0.01~20.0 ng/mL, the linear correlation coefficient (R2) 0.9906. The second part replace the screen printing carbon electrode surface QDs conjugated antibody by ferritin antibody. Detecting human serum ferritin mensurable range is 0.1~20.0 ng/mL, the linear correlation coefficient (R2) 0.9952, detection limit 0.07 ng/mL. Observes in the blood 5 kind of possibly influential species, including: The human serum albumin, Alpha-Fetoprotein, human hemoglobin, human transferrin, ferric chloride. The influence of those species are not serious of our method. To analyze in 10 volunteer blood serum's ferritin result compared with the ELISA method, under 95% confidence degree, those result has good Similarity. Demonstrated that the QDs ECL biosensor sensing ferritin has the high sensitivity, high accuracy, simultaneously cost not expensive, and may produce once a lot.
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