摘要 本研究內容主要進行以液態培養Rhodobacter sphaeroides 生Co-Q10之醱酵研究。由於molasses在過去的實驗中已確定為一良好碳源，因此在三角瓶實驗中，先探討不同氮源的影響性，以及利用NaCl、KCl的添加改變培養基滲透壓實驗，最後探討添加非離子型界面活性劑對於R.sphaeroides生產Co-Q10之影響性。在醱酵槽部份，首先針對氣舉式醱酵槽以及攪拌式醱酵槽在相近的KLa條件下，對於R. sphaeroides生產Co-Q10之影響性，確定生物反應器之後，以饋料為主軸，探討在饋料同時改變溶氧的批次實驗，最後將搖瓶實驗得到最佳添加物的濃度放大至氣舉式醱酵槽作探討。 根據實驗結果顯示，非離子型界面活性劑以添加Tween-80 (6 ml/L) 對於Co-Q10產量增加效果較為明顯， Co-Q10濃度從對照組23.52 mg/L上升到29.75 mg/ L，整體產量提升26%，進一步探討Tween-80各成分添加對於Co-Q10的影響。實驗結果顯示，棕櫚酸添加濃度為3 g/L， Co-Q10產量從對照組23.52 mg/L提升至40.33 mg/L、硬酯酸添加濃度為1 g/L， Co-Q10產量從對照組23.36 mg/L提升至38.42 mg/L，顯示此兩種飽和脂肪酸有助於R. sphaeroides生產Co-Q10。氣舉式醱酵槽部份，實驗結果得知氣舉式醱酵槽以及攪拌式醱酵槽在相近的KLa條件下，氣舉式反應器為較適合生產Co-Q10的生化反應器。 饋料批次實驗證實饋料確實有助於延續菌體生長，但是單位菌體的Co-Q10含量卻明顯降低，因此進行兩階段溶氧饋料批次實驗，由實驗證實饋料同時間降低通氣量有助於Co-Q10生產，在醱酵108小時候得到最佳Co-Q10 產量45.65 mg/L，以及Co-Q10生產速率0.42 mg/L hr。 AbstractThe main of the thesis is using purple non-sulfer bacteria Rhodobacter Sphaeroides to develop a submerged fermentation process for Co-Q10production.Firstly, the effects of different nitrogen sources and screening strategies in flask tests were discussed. After make sure the ferment medium, we discuss the effects of addition non-ion surfactants of Tween series and PEG series, and addition NaCl or KCl to increase medium osmotic. In the fermenter experiment, we use the air-lift fermenter and stirred fermenter in the similar KLa value condition to compare. Make sure the bioreactor then and studying the effect of control strategies by fed-batch were discussed. The results of Tween series adding experiments show Tween-80 (6 ml/L) can increase the Co-Q10 concentraction of control group from 23.52 mg/L reach 29.75 mg/L amounts to 26％, and then we discuss the effects of addition Tween-80 components to medium. The results show addition palmitic acid (3 g/L）can increase the Co-Q10 production of control group from 23.52 mg/L reach 40.33 mg/L, and stearic acid (1 g/L)can increase the Co-Q10 production of control group from 23.36 mg/L reach 38.42 mg/L, these results shows two of saturated fatty acids, palmitic acid and stearic acid can increaseR. sphaeroides production Co-Q10.The results of experiment, air-lift fermenter and stirred fermenter in the similar KLa value condition, air-lift has the better Co-Q10 concentration, shows air-lift is the better bioreactor then stirred fermenter when use R. sphaeroides in ferment process to production Co-Q10. In fed-batch experiment, the result shows this control strategy can increase R. sphaeroides to growth, but specific Co-Q10 content were decrease. A Co-Q10 production of 45.65mg/L and production rate 0.42 mg/L hr were obtained after 108 hr of two steps of control dissolve oxygen concentration in fed-batch culture
