Identification of IgE-binding epitopes and recombinant IgE reactivities of a latex cross-reacting Indian jujube Ziz m 1 allergen.

Tunghai University Institutional Repository > 理學院 > 生命科學系所 > 期刊論文 > Item 310901/27918

Please use this identifier to cite or link to this item: http://140.128.103.80:8080/handle/310901/27918

Title:	Identification of IgE-binding epitopes and recombinant IgE reactivities of a latex cross-reacting Indian jujube Ziz m 1 allergen.
Authors:	黃光裕 Lee, M F Tsai, J J Hwang, G Y Lin, S J Chen, Y H
Contributors:	Taichung Veterans General Hospital Department of Life Science, Tunghai University Chung Shan Medical University National Yang-Ming University
Keywords:	IgE-binding regions Indian jujube latex–fruit syndrome recom-binant Zizm1allergen synthetic peptides
Date:	2008-06
Issue Date:	2016-08-17
Publisher:	USA:Wiley-Blackwell
Abstract:	Zizm1isamajor Indian jujube (Zizyphus mauritiana)allergeninvolvedinlatex-fruit syndrome, and cDNA of the allergen has been cloned, sequencedand expressed in yeast by our laboratory pre viously. In this study, we per-formed an immunoglobulin E (IgE)-binding epitope analysis of Ziz m 1 usingoverlapping recombinant fragments. Eight overlapping recombinant frag-ments were generated from the recombinant Ziz m 1 allergen. The fragmentswere expressed in Escherichia coli and IgE-binding activities were evaluatedby sera of latex–Indian jujube-allergic subjects and normal subjects usingimmunoblotting. Human allergic sera are not able to recognize frag mentsconsisting of amino acid sequences 26–71, 119–280 and 119–291. However,residues at positions 26–199, 26–105, 26–86, 119–320 and 238–330 werefound relevant in the IgE-binding. Our results indicate that72NISGHCSDCTFLGEE86and292VWNRYYDLKTNYSSSIILEYVNSGTKYLP320ofZizm1are the sequences required for human IgE binding. Four correspondingpeptides,72NISGHCSDCTE86,292VWNRYYDLKT301,300KTNYSSSIILEY311and309LEYVNSGTKYLP320, were synthesized, and these peptides reacted with70%, 100%, 70% and 70% of 10 allergic sera tested, as revealed by enzyme-linked immunosorbent assay. Sensitization to292VWNRYYDLKT301correlatedsignificantly with the presence of allergic symptoms (P < 0·001). These find-ings will be useful in designing diagnostic and therapeutic approaches,thereby contributing to the development of specific immunotherapy for sub-jects with latex–fruit syndrome.
Relation:	Clinical and Experimental Immunology, 152(3), 464-471
Appears in Collections:	[生命科學系所] 期刊論文

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	134	View/Open

View Licence

Loading...