北蟲草子實體之抗疲勞功效

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Title:	北蟲草子實體之抗疲勞功效
Other Titles:	Effects of Cordyceps militaris fruiting body on anti-fatigue in ICR mice
Authors:	費皓元 FEI, HAO-YUAN
Contributors:	梁志弘 LIANG, CHIN-HUNG 食品科學系
Keywords:	北蟲草子實體;抗疲勞 Cordyceps militaris fruiting body;anti-fatigue
Date:	2019
Issue Date:	2019-12-16T04:00:59Z (UTC)
Abstract:	疲勞是因勞動或過度工作而產生，與許多職業災害有密切關係。疲勞是指身體無法表現出最佳狀態，為主觀感受、組織損傷或能源短缺所引起。北蟲草（Cordyceps militaris）屬蟲草屬真菌，含蟲草素、腺苷、甘露醣醇及多醣等成份。研究指出北蟲草具有抗糖尿病、降血脂、護肝等多種生理功能。故本研究主要目的為探討北蟲草子實體對運動引起疲勞之改善效果。實驗將八週齡雄性ICR小鼠分為控制組、餵食北蟲草子實體低、中及高劑量組和正控制組等5組，其中正控制組為以通過健康食品認證之冬蟲夏草菌絲體膠囊；每組8隻，餵食四週後，進行運動表現及抗疲勞生化指標分析，運動表現分為前肢拉力及游泳力竭試驗。研究結果顯示，在前肢拉力方面，餵食北蟲草子實體之實驗組(126.62 ~141.86 KgF)及正控制組(146.21 KgF)較控制組(93.85 KgF)有顯著提升；另在游泳力竭實驗方面，餵食北蟲草子實體之實驗組(1267.3~1719.4秒)和正控制組(1405.0秒)之力竭時間比控制組(388.1秒)明顯較長。另抗疲勞血液生化指標方面，研究結果顯示在血糖方面，餵食北蟲草子實體高劑量組可提升1.40倍的血糖含量；在乳酸脫氫酶方面，餵食北蟲草子實體可降低運動後乳酸脫氫酶的生成，低、中和高劑量分別降至1736.3、1541.3和1383.8 U/L，明顯低於控制組(2516.3 U/L)。在血氨方面，餵食北蟲草子實體可降低血氨濃度(565.9~695.4 umol/L)，明顯低於控制組(866.0 umol/L)。在肌酸肌酶方面，餵食北蟲草子實體可降低肌酸肌酶活性，低、中和高劑量分別降至357.5、300.0 及278.8 U/L，明顯低於控制組(903.8 U/L)。此外，在小鼠的肝、腎及其他臟器中並沒有發現病理變化，另在肥胖指數雖各組無顯著差異，但餵食北蟲草子實體，總膽固醇含量及低密度脂蛋白有降低之趨勢。綜上所述，北蟲草子實體可有效提升小鼠前肢拉力，並增加肌耐力，另可減少運動後體內醣原的消耗，降低疲勞產生因子的生成，進而達到抗疲勞之功效。 Fatigue is caused by labor or excessive work, and is closely related to many occupational disasters; therefore, how to reduce the fatigue of workers and occupational injuries has become an important issue. Fatigue refers to the inability of the body to show its optimal state, caused by subjective feelings, tissue damage or energy shortages; the length of the recovery period varies depending on the level of exercise and supplementation of nutrients.Cordyceps militaris is a genus of Cordyceps, containing cordycepin, adenosine, mannitol and polysaccharides. Studies have indicated that Cordyceps militaris has many physiological functions such as anti-diabetes, blood fat reduction and liver protection. At present, among the anti-fatigue functional health products approved by the Food and Drug Administration of the Ministry of Health and Welfare, there are three types of mushroom products, two of which are Cordyceps sinensis mycelium products of Cordyceps. Studies have shown that Cordyceps militaris has more functional components than Cordyceps sinensis. At present, little research has been conducted on the anti-fatigue efficacy of Cordyceps militaris fruiting bodies. Therefore, the main purpose of this study is to investigate the effect of the fruiting body of Cordyceps militaris on exercise-induced fatigue. Eight weeks old male ICR mice were divided into five groups: control group, low, medium and high dose groups of Cordyceps militaris, and positive control group. The positive control group was the Cordyceps sinensis mycelium capsule certified by health food. Eight out of each group, after four weeks of feeding, exercise performance and anti-fatigue biochemical indicators analysis, sports performance is divided into forelimb grip and swimming exhaustion test.The results showed that in the forelimb grip, the experimental group (126.62 ~ 141.86 KgF) and the positive control group (146.21 KgF) fed the Cordyceps militaris fruiting body significantly improved compared with the control group (93.85 KgF); The exhaustion time of the experimental group (1267.3~1719.4 seconds) and the positive control group (1405.0 seconds) fed the Cordyceps militaris fruiting body was significantly longer than that of the control group (388.1 seconds). In addition to anti-fatigue blood biochemical indicators, the results show that in the blood glucose, the high dose group of Cordyceps militaris can increase the blood glucose content by 1.40 times; in the case of lactate dehydrogenase, feeding the Cordyceps militaris fruiting body can reduce the dehydrogenation of lactate after exercise. The enzyme production, low, medium and high doses were reduced to 1736.3, 1541.3 and 1383.8 U / L, respectively, significantly lower than the control group (2516.3 U / L). In terms of blood ammonia, feeding the Cordyceps militaris fruiting body can reduce the blood ammonia concentration (565.9~695.4 umol/L), which is significantly lower than the control group (866.0 umol/L). In terms of creatine kinase, feeding the Cordyceps militaris fruiting body reduced creatine kinase activity, and the low, medium and high doses were reduced to 357.5, 300.0 and 278.8 U/L, respectively, which was significantly lower than the control group (903.8 U/L).In addition, no pathological changes were found in the liver, kidney and other organs of mice. There was no significant difference in the obesity index between the groups, but the total cholesterol content and low-density lipoprotein decreased when fed the Cordyceps militaris fruiting body. In summary, the Cordyceps militaris fruiting body can effectively increase the forelimb grip strength of the mice and increase the muscular endurance. It can also reduce the consumption of glucose in the body after exercise, reduce the production of fatigue-generating factors, and thus achieve the anti-fatigue effect.
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